Til Schlotter
Zürich, Zürich, Schweiz
1170 Follower:innen
500+ Kontakte
Info
Nanotechnology, nanofluidics, biosensing, proteomics, and data analysis
Creative…
Aktivitäten
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Attending the Asilomar Bioelectronics Symposium in California - my favorite place in the U.S. - reminded me of the power of multidisciplinary…
Attending the Asilomar Bioelectronics Symposium in California - my favorite place in the U.S. - reminded me of the power of multidisciplinary…
Beliebt bei Til Schlotter
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We are at Medicine @ETH Day 📣 https://lnkd.in/d5aQDpiy Today, four group leaders from Functional Genomics Center Zurich (FGCZ) – Catharine Aquino,…
We are at Medicine @ETH Day 📣 https://lnkd.in/d5aQDpiy Today, four group leaders from Functional Genomics Center Zurich (FGCZ) – Catharine Aquino,…
Beliebt bei Til Schlotter
Berufserfahrung
Ausbildung
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ETH Zürich
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Dissertation at the Department of Information Technology and Electrical Engineering in the Laboratory of Biosensors and Bioelectronics (Vörös group) on “Interface Nanopores for Single Biomolecule Sensing”
− High-frequent / big data analysis using machine and deep learning approaches
− Micro- and nanofabrication of silicon and polymers for biomolecule sensing
− Experiment design and conduction for single-molecule protein analysis
− Lecturing of biomedical engineering classes and…Dissertation at the Department of Information Technology and Electrical Engineering in the Laboratory of Biosensors and Bioelectronics (Vörös group) on “Interface Nanopores for Single Biomolecule Sensing”
− High-frequent / big data analysis using machine and deep learning approaches
− Micro- and nanofabrication of silicon and polymers for biomolecule sensing
− Experiment design and conduction for single-molecule protein analysis
− Lecturing of biomedical engineering classes and supervision of student projects -
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Reactor Design of an Ion Transport Membrane Combustion
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Master thesis: "Simulation and Design Studies of Catalytic Ionic Transport Membrane Reactors"
Additional courses in Biomedical Engineering -
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Focus on enery, flow, and processes
Bachelor thesis: "Infuence of Process Gas Supply on Particle Size in an Enclosed Flame Spray Pyrolysis Setup" -
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Abschlussarbeit: "China oder Indien? Wer macht das Rennen als Wirtschaftsmacht Nummer eins?"
Bescheinigungen und Zertifikate
Ehrenamt
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Tennistrainer
TC Philatlos (München)
– 1 Jahr 1 Monat
Education
Trainer im Jugendtraining
Veröffentlichungen
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Aptamer-Functionalized Interface Nanopores Enable Amino Acid-Specific Peptide Detection
ACS Nano
Single-molecule proteomics based on nanopore technology has made significant advances in recent years. However, to achieve nanopore sensing with single amino acid resolution, several bottlenecks must be tackled: controlling nanopore sizes with nanoscale precision and slowing molecular translocation events. Herein, we address these challenges by integrating amino acid-specific DNA aptamers into interface nanopores with dynamically tunable pore sizes. A phenylalanine aptamer was used as a…
Single-molecule proteomics based on nanopore technology has made significant advances in recent years. However, to achieve nanopore sensing with single amino acid resolution, several bottlenecks must be tackled: controlling nanopore sizes with nanoscale precision and slowing molecular translocation events. Herein, we address these challenges by integrating amino acid-specific DNA aptamers into interface nanopores with dynamically tunable pore sizes. A phenylalanine aptamer was used as a proof-of-concept: aptamer recognition of phenylalanine moieties led to the retention of specific peptides, slowing translocation speeds. Importantly, while phenylalanine aptamers were isolated against the free amino acid, the aptamers were determined to recognize the combination of the benzyl or phenyl and the carbonyl group in the peptide backbone, enabling binding to specific phenylalanine-containing peptides. We decoupled specific binding between aptamers and phenylalanine-containing peptides from nonspecific interactions (e.g., electrostatics and hydrophobic interactions) using optical waveguide lightmode spectroscopy. Aptamer-modified interface nanopores differentiated peptides containing phenylalanine vs. control peptides with structurally similar amino acids (i.e., tyrosine and tryptophan). When the duration of aptamer–target interactions inside the nanopore were prolonged by lowering the applied voltage, discrete ionic current levels with repetitive motifs were observed. Such reoccurring signatures in the measured signal suggest that the proposed method has the possibility to resolve amino acid-specific aptamer recognition, a step toward single-molecule proteomics.
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Solid-State Nanopores for Biomolecular Analysis and Detection
Springer, Advances in Biochemical Engineering/Biotechnology
Advances in nanopore technology and data processing have rendered DNA sequencing highly accessible, unlocking a new realm of biotechnological opportunities. Commercially available nanopores for DNA sequencing are of biological origin and have certain disadvantages such as having specific environmental requirements to retain functionality. Solid-state nanopores have received increased attention as modular systems with controllable characteristics that enable deployment in non-physiological…
Advances in nanopore technology and data processing have rendered DNA sequencing highly accessible, unlocking a new realm of biotechnological opportunities. Commercially available nanopores for DNA sequencing are of biological origin and have certain disadvantages such as having specific environmental requirements to retain functionality. Solid-state nanopores have received increased attention as modular systems with controllable characteristics that enable deployment in non-physiological milieu. Thus, we focus our review on summarizing recent innovations in the field of solid-state nanopores to envision the future of this technology for biomolecular analysis and detection. We begin by introducing the physical aspects of nanopore measurements ranging from interfacial interactions at pore and electrode surfaces to mass transport of analytes and data analysis of recorded signals. Then, developments in nanopore fabrication and post-processing techniques with the pros and cons of different methodologies are examined. Subsequently, progress to facilitate DNA sequencing using solid-state nanopores is described to assess how this platform is evolving to tackle the more complex challenge of protein sequencing. Beyond sequencing, we highlight the recent developments in biosensing of nucleic acids, proteins, and sugars and conclude with an outlook on the frontiers of nanopore technologies.
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Force-Controlled Formation of Dynamic Nanopores for Single-Biomolecule Sensing and Single-Cell Secretomics
ACS Nano, ACS Publications
Nanopore sensing of single nucleotides has emerged as a promising single-molecule technology for DNA sequencing and proteomics. Despite the conceptual simplicity of nanopores, adoption of this technology for practical applications has been limited by a lack of pore size adjustability and an inability to perform long-term recordings in complex solutions. Here we introduce a method for fast and precise on-demand formation of a nanopore with controllable size between 2 and 20 nm through…
Nanopore sensing of single nucleotides has emerged as a promising single-molecule technology for DNA sequencing and proteomics. Despite the conceptual simplicity of nanopores, adoption of this technology for practical applications has been limited by a lack of pore size adjustability and an inability to perform long-term recordings in complex solutions. Here we introduce a method for fast and precise on-demand formation of a nanopore with controllable size between 2 and 20 nm through force-controlled adjustment of the nanospace formed between the opening of a microfluidic device (made of silicon nitride) and a soft polymeric substrate. The introduced nanopore system enables stable measurements at arbitrary locations. By accurately positioning the nanopore in the proximity of single neurons and continuously recording single-molecule translations over several hours, we have demonstrated this is a powerful approach for single-cell proteomics and secretomics.
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Localized detection of ions and biomolecules with a force-controlled scanning nanopore microscope
Nature Nanotechnology, Springer
Proteins, nucleic acids and ions secreted from single cells are the key signalling factors that determine the interaction of cells with their environment and the neighbouring cells. It is possible to study individual ion channels by pipette clamping, but it is difficult to dynamically monitor the activity of ion channels and transporters across the cellular membrane. Here we show that a solid-state nanopore integrated in an atomic force microscope can be used for the stochastic sensing of…
Proteins, nucleic acids and ions secreted from single cells are the key signalling factors that determine the interaction of cells with their environment and the neighbouring cells. It is possible to study individual ion channels by pipette clamping, but it is difficult to dynamically monitor the activity of ion channels and transporters across the cellular membrane. Here we show that a solid-state nanopore integrated in an atomic force microscope can be used for the stochastic sensing of secreted molecules and the activity of ion channels in arbitrary locations both inside and outside a cell. The translocation of biomolecules and ions through the nanopore is observed in real time in live cells. The versatile nature of this approach allows us to detect specific biomolecules under controlled mechanical confinement and to monitor the ion-channel activities of single cells. Moreover, the nanopore microscope was used to image the surface of the nuclear membrane via high-resolution scanning ion conductance measurements.
Auszeichnungen/Preise
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3rd place PolyPITCH jury vote
Telejob
polyPITCH is a pitch competition for scientific staff at ETH Zurich
https://polypitch.ch/ -
Best Pitch
ETH-EPFL summer school “Sciencepreneurship – from Science to Society”
polyPITCH is a pitch competition for scientific staff at ETH Zurich
https://polypitch.ch/ -
Winner Audience Favourite at PolyPITCH
Telejob
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Best Oral Presentation
Single-Molecule Sensors and nanoSystems International Conference (S3IC)
"Single-Molecule Sensing of Cell Secretion using Force-Controlled Nanopores"
T. Schlotter, S. Weaver, D. Momotenko, J. Vörös, T. Zambelli, M. Aramesh
Single-Molecule Sensors and nanoSystems International Conference (S3IC), 9-11 November 2020, online (planned: Barcelona), Spain -
Best Poster Award
European Biophysics Congress (EBSA)
"Slowdown and detection of nanoparticles and biomolecules using an AFM controlled, cantilever embedded nanopore"
T. Schlotter, J. Vörös, T. Zambelli, M. Aramesh
European Biophysics Congress (EBSA), 20-24 July 2019, Madrid, Spain -
Best Poster Award
Biointerfaces International
"On demand confinement of DNA using an AFM setup with a micro-channelled cantilever"
T. Schlotter, J. Vörös, M. Aramesh
Biointerfaces International, 2018 August 14 - 16, Zurich, Switzerland -
Best Poster Award
From Solid State To Biophysics IX, Dubrovnik, Croatia
"DNA translocation measurements using a modified scanning ion-conductance nanopore setup"
T. Schlotter, J. Vörös, M. Aramesh
From Solid State To Biophysics IX, 2018 June 16 - 22, Dubrovnik, Croatia -
e-fellows.net scholar
foundation of Deutsche Telekom, G. v. Holtzbrinck, McKinsey)
Sprachen
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Deutsch
Muttersprache oder zweisprachig
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Englisch
Verhandlungssicher
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Latein
Grundkenntnisse
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Spanisch
Fließend
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Italienisch
Gute Kenntnisse
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Arabisch
Grundkenntnisse
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Kicking off new innovation in medtech, spacetech, and biotech: dEEGtal, Spacetalk, UNOMR, and Veltist get selected for Venture Kick, receiving CHF…
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