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As$^{\textrm{III}}$ Selectively Induces a Disorder-to-Order Transition in the Metalloid Binding Region of the AfArsR Protein
/ Tóth, Annamária ; Sajdik, Kadosa ; Gyurcsik, Béla ; Nafaee, Zeyad H ; Wéber, Edit ; Kele, Zoltan ; Christensen, Niels Johan ; Schell, Juliana (CERN) ; Correia, Joao Guilherme (CERN) ; Clauss, Kajsa G V Sigfridsson et al.
Arsenic is highly toxic and a significant threat to human health, but certain bacteria have developed defense mechanisms initiated by AsIII binding to AsIII-sensing proteins of the ArsR family. The transcriptional regulator AfArsR responds to AsIII and SbIII by coordinating the metalloids with three cysteines, located in a short sequence of the same monomer chain. [...]
2024 - 14 p.
- Published in : J. Am. Chem. Soc. 146 (2024) 17009-17022
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C‐terminal Cysteines of CueR Act as Auxiliary Metal Site Ligands upon Hg$^{II}$ Binding—A Mechanism To Prevent Transcriptional Activation by Divalent Metal Ions?
/ Balogh, Ria K (Szeged U.) ; Gyurcsik, Béla (Szeged U.) ; Hunyadi‐Gulyás, Éva (Szeged U., Cybernetics Lab) ; Schell, Juliana (CERN ; Duisburg-Essen U.) ; Thulstrup, Peter W (Copenhagen U.) ; Hemmingsen, Lars (Copenhagen U.) ; Jancsó, Attila (Szeged U.)
Intracellular CuI is controlled by the transcriptional regulator CueR, which effectively discriminates between monovalent and divalent metal ions. It is intriguing that HgII does not activate transcription, as bis-thiolate metal sites exhibit high affinity for HgII. [...]
2019 - 6 p.
- Published in : Chemistry – A European Journal 25 (2019) 15030-15035
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A reference compound for $^{199m}$ Hg perturbed angular correlation of $\gamma$ -rays spectroscopy
/ Jancsó, Attila (Szeged U.) ; Correia, Joao G (Lisbon, IST ; CERN) ; Balogh, Ria K (Szeged U.) ; Schell, Juliana (Duisburg-Essen U. ; CERN) ; Jensen, Marianne L (Bohr Inst.) ; Szunyogh, Daniel (Copenhagen U.) ; Thulstrup, Peter W (Copenhagen U.) ; Hemmingsen, Lars (Copenhagen U.)
$^{199m}$ Hg is a very useful probe for perturbed angular correlation of $\gamma$ -rays (PAC) spectroscopy. Here we present $^{199m}$ Hg PAC data for a Hg $^{2+}$ containing compound, Hg(Cys)$_{2}$(s), giving one unique nuclear quadrupole interaction (NQI). [...]
2021
- Published in : Nucl. Instrum. Methods Phys. Res., A 1002 (2021) 165154
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Towards $^{31}$Mg-$\beta$-NMR resonance linewidths adequate for applications in magnesium chemistry
/ Stachura, M (TRIUMF) ; McFadden, R M L (British Columbia U., Chem. Dept.) ; Chatzichristos, A (British Columbia U.) ; Dehn, M H (British Columbia U.) ; Gottberg, A (TRIUMF) ; Hemmingsen, L (U. Copenhagen (main)) ; Jancso, A (Szeged U.) ; Karner, V L (British Columbia U., Chem. Dept.) ; Kiefl, R F (British Columbia U.) ; Larsen, F H (U. Copenhagen (main)) et al.
The span of most chemical shifts recorded in conventional $^{25}$Mg-NMR spectroscopy is $\sim$ 100 ppm. Accordingly, linewidths of $\sim$ 10 ppm or better are desirable to achieve adequate resolution for applications in chemistry. [...]
2017 - 8 p.
- Published in : Hyperfine Interact. 238 (2017) 32
In : 10th International Workshop on Application of Lasers and Storage Devices in Atomic Nuclei Research : Recent Achievements and Future Prospects, Poznan, Poland, 16 - 19 May 2016, pp.32
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Specificity of the Metalloregulator CueR for Monovalent Metal Ions: Possible Functional Role of a Coordinated Thiol?
/ Szunyogh, Dániel (MTA‐SZTE Bioinorganic Chemistry Research Group, Hungary) ; Szokolai, Hajnalka (Szeged U.) ; Thulstrup, Peter W (Copenhagen U.) ; Larsen, Flemming H (Copenhagen U.) ; Gyurcsik, Béla (Szeged U.) ; Christensen, Niels Johan (Copenhagen U.) ; Stachura, Monika (CERN ; TRIUMF) ; Hemmingsen, Lars (Copenhagen U.) ; Jancsó, Attila (Szeged U.)
Metal-ion-responsive transcriptional regulators within the MerR family effectively discriminate between mono- and divalent metal ions.Herein we address the origin of the specificity of the CueR protein for monovalent metal ions.Several spectroscopic techniques were employed to study $Ag^I$ ,$Zn^{II}$ ,and $Hg^{II}$ binding to model systems encompassing the metal-ion-binding loop of CueR from E. coli and V. [...]
2015 - 6 p.
- Published in : Angew. Chem. Int. Ed. 54 (2015) 15756-15761
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Cu(I), Ag(I), Cd(II), Hg(II), and Pb(II) binding to biomolecules studied by perturbed angular correlation (PAC) of $\gamma$-rays spectroscopy
/ Hemmingsen, L (Department of Chemistry, University of Copenhagen, Denmark) ; Thulstrup, P W (Department of Chemistry, University of Copenhagen, Denmark) ; Pecoraro, V L (Department of Chemistry, University of Michigan, USA) ; Jancso, A (Department of Inorganic and Analytical Chemistry, University of Szeged, Hungary) ; Muller, J (Department of Inorganic and Analytical Chemistry, Westfälische Wilhelms-Universität Münster, Germany) ; Johnston, K (ISOLDE, CERN) ; Stachura, M (ISOLDE, CERN) ; Correia, J G (ISOLDE, CERN)
CERN-INTC-2014-063 ; INTC-P-427.
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2014.
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β-NMR of copper isotopes in ionic liquids
/ Stachura, Monika (CERN) ; Hemmingsen, Lars (Department of Chemistry, University of Copenhagen, Denmark) ; Gottberg, Alexander (CERN) ; Kowalska, Magdalena (CERN) ; Bissell, Mark L (Institute for Nuclear and Radiation Physics, KU Leuven, Belgium) ; Bjerrum, Morten J (Department of Chemistry, University of Copenhagen, Denmark) ; Flanagan, Kieran (School of Physics and Astronomy, University of Manchester, United Kingdom) ; Garcia Ruiz, Ronald F (Institute for Nuclear and Radiation Physics, KU Leuven, Belgium) ; Georgiev, Georgi (CSNSM, Orsay, France) ; Jancso, Attila (Department of Chemistry, University of Szeged, Hungary) et al.
CERN-INTC-2013-034 ; INTC-P-393.
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2013.
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$^{31}$Mg $\beta$-NMR applied in chemistry and biochemistry
/ Stachura, Monika (CERN) ; Gottberg, Alexander (CERN) ; Kowalska, Magdalena (CERN) ; Johnston, Karl (CERN) ; Bissell, Mark L (Nuclear and Radiation Physics Section, KU Leuven, Belgium) ; Garcia Ruiz, Ronald F (Nuclear and Radiation Physics Section, KU Leuven, Belgium) ; Georgiev, Georgi (CSNSM, Orsay, France) ; Luczkowski, Marek (Department of Chemistry, University of Wroclaw, Poland) ; Neyens, Gerda (Nuclear and Radiation Physics Section, KU Leuven, Belgium) ; Kiefl, Robert F (Department of Chemistry, University of British Columbia, Canada) et al.
CERN-INTC-2013-033 ; INTC-P-392.
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2013.
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