For the
SPME analysis, samples from fresh and drying leaves were cut, and 2.0g of the samples was placed in a glass vial containing 5mL of the water and 3g of NaCl.
For
SPME passive sampling, the method by Pacenti et al.
After drying the plant materials in room temperature (25 AdegC), flower and leaf samples were subjected to solid phase microextraction (
SPME, Supelco, Germany) with a fibre precoated with a 75 um-thick layer of Carboxen/Polydimethylsiloxane (CAR/PDMS).
Since then, nine different classes of compounds (alcohols, aldehydes, ketones and terpenes) have been identified using
SPME in at least 60 termite species (Bordereau & Pasteels, 2010; Cristaldo et al., 2014).
In recent years, the emergence of many kinds of materials has brought new opportunities for the development of
SPME fiber coating.
A total of 96 volatile compounds were detected by GC-MS coupled with
SPME (supplementary Table S1) and were subdivided into different classes, including 12 acids, 17 alcohols, 23 aldehydes, 33 esters, four ketones, three nitrogenous compounds, and four lactones.
The small size and biocompatibility of
SPME materials also permits in vivo sampling of saliva (Bessonneau et al.
Volatile compounds were extracted by
SPME (Supelco, Bellefonte, PA, USA) and subsequently analysed by gas chromatography coupled with mass spectrometry (GC-MS) (Agilent Technologies, Palo Alto, CA, USA).
* Full analytical support for lubricant and plasticizer development and modern analysis including NMR, FTIR,
SPME, GC/MS, HPLC, HPLC/MS, DSC TGA and viscosity measurement as well as physical testing to measure percentage elongation, compression and tensile strength.
Extraction of Volatiles of Fire Ant Workers by Solid Phase Microextraction (
SPME).