Abstract
GADD45 is a ubiquitously expressed mammalian gene that is induced by DNA damage and certain other stresses. Like another p53-regulated gene, p21WAF1/CIP1, whose product binds to cyclin-dependent kinases (Cdk's) and proliferating cell nuclear antigen (PCNA), GADD45 has been associated with growth suppression. Gadd45 was found to bind to PCNA, a normal component of Cdk complexes and a protein involved in DNA replication and repair. Gadd45 stimulated DNA excision repair in vitro and inhibited entry of cells into S phase. These results establish GADD45 as a link between the p53-dependent cell cycle checkpoint and DNA repair.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cell Division / drug effects
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Cell Line
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Cyclin-Dependent Kinase Inhibitor p21
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Cyclins / metabolism
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DNA / biosynthesis
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DNA Damage
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DNA Repair*
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GADD45 Proteins
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Genes, p53*
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Humans
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Intracellular Signaling Peptides and Proteins
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Proliferating Cell Nuclear Antigen / metabolism*
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Proteins / metabolism*
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Proteins / pharmacology
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Recombinant Proteins / metabolism
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Recombinant Proteins / pharmacology
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S Phase / drug effects*
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Transfection
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Tumor Cells, Cultured
Substances
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CDKN1A protein, human
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Cyclin-Dependent Kinase Inhibitor p21
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Cyclins
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Intracellular Signaling Peptides and Proteins
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Proliferating Cell Nuclear Antigen
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Proteins
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Recombinant Proteins
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DNA