Investigation of gallic acid induced anticancer effect in human breast carcinoma MCF-7 cells

J Biochem Mol Toxicol. 2014 Sep;28(9):387-93. doi: 10.1002/jbt.21575. Epub 2014 May 27.

Abstract

Gallic acid (GA), a polyhydroxylphenolic compound abundantly distributed in plants, fruits, and foods, has been reported to have various biological activities including an anticancer effect. In this study, we extensively investigated the anticancer effect of GA in human breast carcinoma MCF-7 cells. Our study indicated that treatment with GA resulted in inhibition of proliferation and induction of apoptosis in MCF-7 cells. Then, the molecular mechanism of GA's apoptotic action in MCF-7 cells was further investigated. The results revealed that GA induced apoptosis by triggering the extrinsic or Fas/FasL pathway as well as the intrinsic or mitochondrial pathway. Furthermore, the apoptotic signaling induced by GA was amplified by cross-link between the two pathways. Taken together, our findings may be useful for understanding the mechanism of action of GA on breast cancer cells and provide new insights into the possible application of such compound and its derivatives in breast cancer therapy.

Keywords: Cell Apoptosis; Fas/FasL Pathway; Gallic Acid; MCF-7; Mitochondrial Pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects*
  • Breast Neoplasms / drug therapy*
  • Breast Neoplasms / genetics
  • Breast Neoplasms / metabolism
  • Cell Line, Tumor
  • Cell Proliferation / drug effects*
  • Fas Ligand Protein / genetics
  • Fas Ligand Protein / metabolism
  • Female
  • Gallic Acid / pharmacology*
  • Humans
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / metabolism
  • fas Receptor / genetics
  • fas Receptor / metabolism

Substances

  • Antineoplastic Agents
  • FAS protein, human
  • FASLG protein, human
  • Fas Ligand Protein
  • Neoplasm Proteins
  • fas Receptor
  • Gallic Acid