lnterleukin-8 gene polymorphism and susceptibility to gastric cancer in a Brazilian population

Biol Res. 2012;45(4):369-74. doi: 10.4067/S0716-97602012000400007.

Abstract

Background: Studies have demonstrated that some polymorphisms in different interleukin genes may increase the risk of cancer. The aim of this study was to investigate the association between the IL-8 (rs4073) -251A/T gene polymorphism and the risk of gastric cancer (GC).

Patients and methods: A case-control study was conducted on patients with noncardia gastric cancer. DNA was extracted from leukocytes and the IL-8 (rs4073) -251A/T polymorphism was analyzed by PCR-RFLP. Infection with Helicobacter pylori was investigated in the serum by ELISA.

Results: The sample consisted of 104 patients with GC and 196 controls. Cigarette smoking (P=0.007) and high fat intake (P=0.01) were more frequent in patients with GC. The proportion of patients infected with H. pylori was similar in the two groups (P=0.101). The frequency of the genotype A/T was higher in the cancer group (P=0.008). An increased risk of GC was found in subjects carrying the genotype A/T (OR=2.50, CI: 1.27-4.90), subjects with high fat intake (OR=1.92, CI: 1.17-3.15), and smokers (OR=2.00, CI: 1.203.31).

Conclusions: Subjects with the heterozygous A/T genotype, high fat intake and smokers or ex-smokers presented an increased risk of GC. Individuals with A/A genotype may have protective effect for GC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brazil
  • Case-Control Studies
  • Female
  • Genetic Predisposition to Disease*
  • Genotype
  • Helicobacter Infections / complications
  • Helicobacter pylori / immunology
  • Humans
  • Interleukin-8 / genetics*
  • Kaplan-Meier Estimate
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Polymorphism, Restriction Fragment Length
  • Risk Factors
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / microbiology

Substances

  • Interleukin-8