Telomerase activation induces elongation of the telomeric single-stranded overhang, but does not prevent chromosome aberrations in human vascular endothelial cells

Kumiko Anno; Akane Hayashi; Tomoko Takahashi; Youji Mitsui; Toshinori Ide; Hidetoshi Tahara

doi:10.1016/j.bbrc.2006.12.112

Telomerase activation induces elongation of the telomeric single-stranded overhang, but does not prevent chromosome aberrations in human vascular endothelial cells

Biochem Biophys Res Commun. 2007 Feb 23;353(4):926-32. doi: 10.1016/j.bbrc.2006.12.112. Epub 2006 Dec 22.

Authors

Kumiko Anno¹, Akane Hayashi, Tomoko Takahashi, Youji Mitsui, Toshinori Ide, Hidetoshi Tahara

Affiliation

¹ Department of Cellular and Molecular Biology, Division of Integrated Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8553, Japan.

PMID: 17207773
DOI: 10.1016/j.bbrc.2006.12.112

Abstract

Chromosome aberrations such as loss of chromosome 13 were frequently observed in human endothelial cells from umbilical cord veins (HUVEC). A recent study showed that the length of telomeric single-stranded 3'-overhangs (G-tails) is more important as an essential structure for chromosome maintenance than the net telomere length in telomere t-loop formation. Here, we have examined G-tail length using G-tail telomere HPA in normal and hTERT-transduced HUVECs. We found that forced expression of hTERT in HUVEC induced G-tail as well as total telomere length elongation. G-tail length was well correlated with total telomere length. However, hTERT introduction did not prevent chromosome aberrations such as loss of chromosome 13. Normal characteristics such as morphology, up-regulation of vWF, and tube formation were observed in hTERT-HUVEC as in young normal HUVEC. These results show that chromosome aberrations in HUVEC are independent of telomere G-tail and total telomere attrition.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blotting, Western
Cell Proliferation
Cells, Cultured
Cellular Senescence / genetics
Chromosome Aberrations*
DNA, Single-Stranded / chemistry
DNA, Single-Stranded / genetics*
Endothelial Cells / cytology
Endothelial Cells / metabolism*
Fibroblast Growth Factor 2 / genetics
Fibroblast Growth Factor 2 / metabolism
Gene Expression Profiling
Gene Expression Regulation, Enzymologic
Genetic Vectors / genetics
HeLa Cells
Humans
Immunohistochemistry
Karyotyping
Microscopy, Fluorescence
RNA, Messenger / genetics
RNA, Messenger / metabolism
Telomerase / genetics
Telomerase / metabolism*
Telomere / genetics*
Telomere / metabolism
Transfection
Vascular Endothelial Growth Factor A / genetics
Vascular Endothelial Growth Factor A / metabolism
von Willebrand Factor / genetics
von Willebrand Factor / metabolism

Substances

DNA, Single-Stranded
RNA, Messenger
Vascular Endothelial Growth Factor A
von Willebrand Factor
Fibroblast Growth Factor 2
Telomerase