Cloning and sequence analysis of dsRNA segments 5, 6 and 7 of a novel non-group A, B, C adult rotavirus that caused an outbreak of gastroenteritis in China

Virus Res. 2004 Nov;106(1):15-26. doi: 10.1016/j.virusres.2004.05.011.

Abstract

A diarrhoeal outbreak among adults in China was caused by a new rotavirus, termed ADRV-N, that does not react with antisera directed against group A, B or C rotaviruses [Zhonghua Liu Xing Bing Xue Za Zhi (Chin. Epidemiol.) 19 (1998) 336]. ADRV-N can be propagated in cell cultures [Zhonghua Yi Xue Za Zhi (Natl. Med. J. China) 82 (2002) 14]. We present the complete sequences for ADRV-N genome segments 5 and 6, and a full ORF sequence of genome segment 7. The deduced amino acid sequences suggest that these segments encode NSP1, VP6 and NSP3, respectively. These three ADRV-N genome segments have a unique -ACCCC-3' terminal sequence. The 5'-GG- terminus of segments 5 and 6 is the same as that of other rotaviruses. The amino acid similarity between VP6 and NSP3 of ADRV-N and the cognate sequences of their closest counterpart, group B IDIR, was 37 and 35%, respectively. The ADRV-N NSP1 has a double-stranded RNA binding motif (DSRM) and a putative autoproteolytic cleavage motif upstream from the DSRM. The putative ADRV-N NSP3 has a truncated C-terminus compared to the cognate protein of group B rotaviruses. All the available data demonstrate that ADRV-N differs significantly from the known rotaviruses and strongly suggest that ADRV-N is the first recognized member of a new group of rotaviruses infecting humans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Capsid Proteins / genetics
  • China
  • Cloning, Molecular
  • Disease Outbreaks*
  • Gastroenteritis / epidemiology*
  • Gastroenteritis / virology
  • Genome, Viral
  • Humans
  • Phylogeny
  • RNA, Double-Stranded / analysis
  • RNA, Viral / analysis*
  • RNA, Viral / genetics
  • Rotavirus / chemistry
  • Rotavirus / classification
  • Rotavirus / genetics*
  • Rotavirus Infections / epidemiology*
  • Rotavirus Infections / virology
  • Sequence Analysis

Substances

  • Capsid Proteins
  • RNA, Double-Stranded
  • RNA, Viral