Analyses of 123 Peripheral Human Immune Cell Subsets: Defining Differences with Age and between Healthy Donors and Cancer Patients Not Detected in Analysis of Standard Immune Cell Types

doi:10.5772/62322

PMC full text:

J Circ Biomark. 2016 Jan-Dec; 5: 5.

Published online 2016 Mar 10. doi: 10.5772/62322

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Table 2.

Standard parental immune cell types in healthy donors under and over age 40

Immune cell type	Age <40	Age >40	P value	Direction of age >40
CD8⁺ T cells	19	8	0.0009	↓
NK	5	10	0.0040	↑
CD4⁺ T cells	34	29	0.8975	=
Tregs	1.4	1.5	0.9999	=
B cells	11	16	0.1645	=
NK-T	2.4	1.3	0.4116	=
cDC	0.4	0.3	0.9999	=
pDC	0.2	0.2	0.8975	=
MDSC	5	4	0.9999	=

A total of 9 standard parental immune cell types were analyzed. Values are displayed as median % of PBMCs. P-value was calculated with the Mann Whitney test with Holm adjustment made for multiple comparisons using the number of standard immune cell types with frequency above 0.01% of PBMCs (n=9). Differences were defined as subsets with an adjusted p<0.05, medians at least 50% different, and frequency above 0.01% of PBMCs.

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cDC, conventional dendritic cells; MDSC, myeloid derived suppressor cells; NK, natural killer; PBMCs, peripheral blood mononuclear cells; pDC, plasmacytoid DC; Tregs, regulatory T cells.