The real-time alignment and calibration procedure is a fully automatic procedure at LHCb that is executed within each fill of the LHC. This allows identical alignment and calibration constants to be used in the online and offline reconstruction, ensuring consistency between triggered and offline selected events. The alignment estimates the position of detector elements and is essential to achieve the best data quality. The procedure is implemented for the full LHCb tracking system and the event reconstruction is run using multithreaded processes. The operational and technical aspects of this procedure during data-taking is discussed with the focus on the performance in the 2024 data-taking period where the first global tracker alignment was obtained.