Overcoming barriers to membrane protein structure determination. (Q30401692): Difference between revisions

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scientific article

Language	Label	Description	Also known as
English	Overcoming barriers to membrane protein structure determination.	scientific article

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scholarly article

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Europe PubMed Central

PubMed publication ID

21 June 2017

Overcoming barriers to membrane protein structure determination. (English)

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21 June 2017

protein structure

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7

object named as

Simon Newstead

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21 June 2017

author name string

Roslyn M Bill

1

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21 June 2017

Peter J F Henderson

2

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21 June 2017

So Iwata

3

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21 June 2017

Edmund R S Kunji

4

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21 June 2017

Hartmut Michel

5

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21 June 2017

Richard Neutze

6

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21 June 2017

Bert Poolman

8

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21 June 2017

Christopher G Tate

9

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21 June 2017

Horst Vogel

10

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21 June 2017

publication date

1 April 2011

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21 June 2017

Nature Biotechnology

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21 June 2017

29

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4

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335-340

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21 June 2017

Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy

1 reference

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High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor

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Crystal structure of squid rhodopsin

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Crystal structure of the ligand-free G-protein-coupled receptor opsin

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Crystal structure of the human beta2 adrenergic G-protein-coupled receptor

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GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function

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Crystal structure of opsin in its G-protein-interacting conformation

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The structural basis of calcium transport by the calcium pump

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Crystal structure of the sodium-potassium pump at 2.4 A resolution

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Structure and mechanism of an amino acid antiporter

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Molecular basis of transport and regulation in the Na(+)/betaine symporter BetP

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Structure and Mechanism of a Na+-Independent Amino Acid Transporter

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Molecular Basis of Alternating Access Membrane Transport by the Sodium-Hydantoin Transporter Mhp1

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The alternating access mechanism of transport as observed in the sodium-hydantoin transporter Mhp1.

1 reference

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Structure of a potentially open state of a proton-activated pentameric ligand-gated ion channel

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Making membrane proteins for structures: a trillion tiny tweaks

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Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

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Tuning Escherichia coli for membrane protein overexpression

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Overexpression of integral membrane proteins for structural studies

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Overexpression of mammalian integral membrane proteins for structural studies.

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Purification of a rat neurotensin receptor expressed in Escherichia coli

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Export from the endoplasmic reticulum represents the limiting step in the maturation and cell surface expression of the human delta opioid receptor

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Heterologous expression of G-protein-coupled receptors: comparison of expression systems from the standpoint of large-scale production and purification.

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Molecular chaperones stimulate the functional expression of the cocaine-sensitive serotonin transporter

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Calnexin co-expression and the use of weaker promoters increase the expression of correctly assembled Shaker potassium channel in insect cells

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Evolved Lactococcus lactis strains for enhanced expression of recombinant membrane proteins.

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Consequences of membrane protein overexpression in Escherichia coli.

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Design of improved membrane protein production experiments: quantitation of the host response

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Relieving the first bottleneck in the drug discovery pipeline: using array technologies to rationalize membrane protein production.

1 reference

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Genome sequence of the recombinant protein production host Pichia pastoris

1 reference

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Large-scale functional expression of WT and truncated human adenosine A2A receptor in Pichia pastoris bioreactor cultures

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Stress in recombinant protein producing yeasts.

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Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory

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Lactococcus lactis as host for overproduction of functional membrane proteins

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Comparative analysis and "expression space" coverage of the production of prokaryotic membrane proteins for structural genomics.

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Selenomethionine incorporation in proteins expressed inLactococcus lactis

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Amino acid accumulation limits the overexpression of proteins in Lactococcus lactis

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The structural basis for peptide selection by the transport receptor OppA

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Optimization of membrane protein overexpression and purification using GFP fusions.

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A scalable, GFP-based pipeline for membrane protein overexpression screening and purification

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Quality control of overexpressed membrane proteins

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High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae

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Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins

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Visualizing odorant receptor trafficking in living cells down to the single-molecule level

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FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells

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Probing the function of ionotropic and G protein-coupled receptors in surface-confined membranes.

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Engineering G protein-coupled receptors to facilitate their structure determination.

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Crystal structure of rhodopsin: A G protein-coupled receptor

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Crystallizing membrane proteins using lipidic mesophases

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Purification and characterization of the human adenosine A(2a) receptor functionally expressed in Escherichia coli

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Determination of the molecular mass and dimensions of membrane proteins by size exclusion chromatography

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Building a thermostable membrane protein

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Co-evolving stability and conformational homogeneity of the human adenosine A2a receptor

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Thermostabilization of the neurotensin receptor NTS1.

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Development and crystallization of a minimal thermostabilised G protein-coupled receptor.

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A Specific Cholesterol Binding Site Is Established by the 2.8 Å Structure of the Human β2-Adrenergic Receptor

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Structure of a beta1-adrenergic G-protein-coupled receptor

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Protein conformational changes in the bacteriorhodopsin photocycle: comparison of findings from electron and X-ray crystallographic analyses.

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Protein crystallography with a micrometre-sized synchrotron-radiation beam

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Structure at 2.8 Å resolution of cytochrome c oxidase from Paracoccus denitrificans

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Structural dynamics of light-driven proton pumps

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Yeast mitochondrial ADP/ATP carriers are monomeric in detergents

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Identifiers

10.1038/NBT.1833

1 reference

Europe PubMed Central

PubMed publication ID

21 June 2017

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

21 June 2017

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