Version 1
: Received: 13 June 2023 / Approved: 14 June 2023 / Online: 14 June 2023 (07:21:24 CEST)
How to cite:
Goedicke-Fritz, S.; Bous, M.; Weber, R.; Kaiser, E.; Rogosch, T.; Markert, S.; Kerzel, S.; Schmidt, T.; Maier, R. F.; Zemlin, M. Frequency of Circulating B Cell Subpopulations in Umbilical Cord Blood and Adult Venous Blood. Preprints2023, 2023060989. https://doi.org/10.20944/preprints202306.0989.v1
Goedicke-Fritz, S.; Bous, M.; Weber, R.; Kaiser, E.; Rogosch, T.; Markert, S.; Kerzel, S.; Schmidt, T.; Maier, R. F.; Zemlin, M. Frequency of Circulating B Cell Subpopulations in Umbilical Cord Blood and Adult Venous Blood. Preprints 2023, 2023060989. https://doi.org/10.20944/preprints202306.0989.v1
Goedicke-Fritz, S.; Bous, M.; Weber, R.; Kaiser, E.; Rogosch, T.; Markert, S.; Kerzel, S.; Schmidt, T.; Maier, R. F.; Zemlin, M. Frequency of Circulating B Cell Subpopulations in Umbilical Cord Blood and Adult Venous Blood. Preprints2023, 2023060989. https://doi.org/10.20944/preprints202306.0989.v1
APA Style
Goedicke-Fritz, S., Bous, M., Weber, R., Kaiser, E., Rogosch, T., Markert, S., Kerzel, S., Schmidt, T., Maier, R. F., & Zemlin, M. (2023). Frequency of Circulating B Cell Subpopulations in Umbilical Cord Blood and Adult Venous Blood. Preprints. https://doi.org/10.20944/preprints202306.0989.v1
Chicago/Turabian Style
Goedicke-Fritz, S., Rolf Felix Maier and Michael Zemlin. 2023 "Frequency of Circulating B Cell Subpopulations in Umbilical Cord Blood and Adult Venous Blood" Preprints. https://doi.org/10.20944/preprints202306.0989.v1
Abstract
Background: The intrauterine and early extrauterine development represents a “window of opportunity” in the immunological development. The underlying mechanisms are still poorly understood. The aim of this study was to provide reference values B cell subpopulations in cord blood of term newborns, juveniles and in adults to find the spectrum of their physiological age-related variation.
Methods: In this study, we used flow cytometry to evaluate human B lymphocytes and subpopulations in cord blood (n = 10), in peripheral blood from healthy juveniles aged 1 to 17 years (n=20) and from donors aged 24 to 62 years (n = 10).
Results: Our findings showed increasing frequencies of IgM memory B cells, class-switched memory B cells, marginal zone B cells and plasmablasts, from cord blood to peripheral blood of juveniles and adults. In con-trast, the percentage of naïve B cells was higher in newborns than in juveniles and adults. The frequencies of were similar in cord blood and peripheral blood of adults. Interestingly, transitional B cells frequencies were similar in cord blood and adults but significantly lower in juveniles.
Conclusions: The frequencies of circulating B cell subpopulation are subject to considerable changes during on-togeny, reflecting overlying effects of maturation and of the acquisition of an adaptive immune memory.
Keywords
B cells; B1 cells; human; leukocytes; cord blood; development; ontogeny
Subject
Medicine and Pharmacology, Immunology and Allergy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.