NOX2

NADPH oksidaz 2 veya sitokrom b(558) alt-birimi beta veya Sitokrom B-245 ağır zincir, insanlarda NOX2 geni (diğer adıyla CYBB geni) tarafından kodlanan bir proteindir.^[1] Bu protein, reaktif oksijen türlerini (ROS) oluşturan süper oksiti üreten bir enzimdir.

Fonksiyon

Nox2 veya Sitokrom b (-245), sitokrom b alfa (CYBA) ve beta (CYBB) zincirinden oluşur. Fagositlerin mikrobisidal oksidaz sisteminin birincil bileşeni olduğu düşünülür.

Nox2, NADPH oksidazın katalitik, zara bağlı alt birimidir. Flavositokrom b558 olarak bilinen heterodimeri oluşturan membrana sabitlenmiş p22 <i id="mwKA">phox'a</i> bağlanana kadar inaktiftir.^[2] Aktivasyondan sonra, düzenleyici alt birimler p67phox, p47phox, p40phox ve tipik bir Rac olan GTPaz, plazma membranı veya fagozomal membran üzerinde NADPH oksidaz oluşturmak için komplekse dahil edilir.^[3] Nox2'nin kendisi, iki hem grubunu bağlayan bir N-terminal transmembran domaininden ve FAD ve NADPH'ye bağlanabilen bir C-terminal domaininden oluşur.^[4]

Kanıtlar, bu proteinin aortik ark, torasik ve abdominal aortta aterosklerotik lezyon gelişiminde önemli bir rol oynadığını göstermiştir.^[5]^[6]

Etkileşimler

NoX2 Podosit TRPC6 kanalları ile doğrudan etkileşir.^[7]

Kaynakça

^ "Entrez Gene: CYBB cytochrome b-245, beta polypeptide (chronic granulomatous disease)". 2 Kasım 2008 tarihinde kaynağından arşivlendi.
^ "NADPH oxidases in Eukaryotes: red algae provide new hints!". Current Genetics. 49 (3): 190-204. Mart 2006. doi:10.1007/s00294-005-0044-z. PMID 16344959.
^ "Molecular evolution of Phox-related regulatory subunits for NADPH oxidase enzymes". BMC Evolutionary Biology. 7: 178. Eylül 2007. doi:10.1186/1471-2148-7-178. PMC 2121648 $2. PMID 17900370.
^ Aguirre (2010). "Nox enzymes from fungus to fly to fish and what they tell us about Nox function in mammals". Free Radical Biology and Medicine. 49 (9): 1342-1353. doi:10.1016/j.freeradbiomed.2010.07.027. PMC 2981133 $2. PMID 20696238.
^ Sorescu (2002). "Superoxide production and expression of nox family proteins in human atherosclerosis". Circulation. 105 (12): 1429-35. doi:10.1161/01.cir.0000012917.74432.66. PMID 11914250.
^ Chaubey (2013). "Nox2 is required for macrophage chemotaxis towards CSF-1". PLOS ONE. 8 (2): e54869. doi:10.1371/journal.pone.0054869. PMC 3562318 $2. PMID 23383302.
^ "NOX2 interacts with podocyte TRPC6 channels and contributes to their activation by diacylglycerol: essential role of podocin in formation of this complex". American Journal of Physiology. Cell Physiology. 305 (9): C960-71. Kasım 2013. doi:10.1152/ajpcell.00191.2013. PMID 23948707.

Konuyla ilgili yayınlar

Bolscher BG, de Boer M, de Klein A, Weening RS, Roos D (Haziran 1991). "Point mutations in the beta-subunit of cytochrome b558 leading to X-linked chronic granulomatous disease". Blood. 77 (11): 2482-7. doi:10.1182/blood.V77.11.2482.2482. PMID 1710153.
Nong Y, Kandil O, Tobin EH, Rose RM, Remold HG (Ocak 1991). "The HIV core protein p24 inhibits interferon-gamma-induced increase of HLA-DR and cytochrome b heavy chain mRNA levels in the human monocyte-like cell line THP1". Cellular Immunology. 132 (1): 10-6. doi:10.1016/0008-8749(91)90002-S. PMID 1905983.
Dinauer MC, Pierce EA, Bruns GA, Curnutte JT, Orkin SH (Kasım 1990). "Human neutrophil cytochrome b light chain (p22-phox). Gene structure, chromosomal location, and mutations in cytochrome-negative autosomal recessive chronic granulomatous disease". The Journal of Clinical Investigation. 86 (5): 1729-37. doi:10.1172/JCI114898. PMC 296926 $2. PMID 2243141.
Royer-Pokora B, Kunkel LM, Monaco AP, Goff SC, Newburger PE, Baehner RL, Cole FS, Curnutte JT, Orkin SH (1986). "Cloning the gene for an inherited human disorder--chronic granulomatous disease--on the basis of its chromosomal location" (PDF). Nature. 322 (6074): 32-8. doi:10.1038/322032a0. hdl:2027.42/62926. PMID 2425263. 30 Mart 2012 tarihinde kaynağından arşivlendi (PDF).
Dinauer MC, Curnutte JT, Rosen H, Orkin SH (Aralık 1989). "A missense mutation in the neutrophil cytochrome b heavy chain in cytochrome-positive X-linked chronic granulomatous disease". The Journal of Clinical Investigation. 84 (6): 2012-6. doi:10.1172/JCI114393. PMC 304086 $2. PMID 2556453.
Dinauer MC, Orkin SH, Brown R, Jesaitis AJ, Parkos CA (1987). "The glycoprotein encoded by the X-linked chronic granulomatous disease locus is a component of the neutrophil cytochrome b complex". Nature. 327 (6124): 717-20. doi:10.1038/327717a0. PMID 3600768.
Teahan C, Rowe P, Parker P, Totty N, Segal AW (1987). "The X-linked chronic granulomatous disease gene codes for the beta-chain of cytochrome b-245". Nature. 327 (6124): 720-1. doi:10.1038/327720a0. PMID 3600769.
Rabbani H, de Boer M, Ahlin A, Sundin U, Elinder G, Hammarström L, Palmblad J, Smith CI, Roos D (Ekim 1993). "A 40-base-pair duplication in the gp91-phox gene leading to X-linked chronic granulomatous disease". European Journal of Haematology. 51 (4): 218-22. doi:10.1111/j.1600-0609.1993.tb00634.x. PMID 7694872.
Pollock JD, Williams DA, Gifford MA, Li LL, Du X, Fisherman J, Orkin SH, Doerschuk CM, Dinauer MC (Şubat 1995). "Mouse model of X-linked chronic granulomatous disease, an inherited defect in phagocyte superoxide production". Nature Genetics. 9 (2): 202-9. doi:10.1038/ng0295-202. PMID 7719350.
Ariga T, Sakiyama Y, Matsumoto S (Ekim 1994). "Two novel point mutations in the cytochrome b 558 heavy chain gene, detected in two Japanese patients with X-linked chronic granulomatous disease". Human Genetics. 94 (4): 441. doi:10.1007/BF00201609. PMID 7927345.
Leto TL, Adams AG, de Mendez I (Ekim 1994). "Assembly of the phagocyte NADPH oxidase: binding of Src homology 3 domains to proline-rich targets". Proceedings of the National Academy of Sciences of the United States of America. 91 (22): 10650-4. doi:10.1073/pnas.91.22.10650. PMC 45079 $2. PMID 7938008.
Ariga T, Sakiyama Y, Tomizawa K, Imajoh-Ohmi S, Kanegasaki S, Matsumoto S (Haziran 1993). "A newly recognized point mutation in the cytochrome b558 heavy chain gene replacing alanine57 by glutamic acid, in a patient with cytochrome b positive X-linked chronic granulomatous disease". European Journal of Pediatrics. 152 (6): 469-72. doi:10.1007/BF01955051. PMID 8101486.
Leusen JH, de Boer M, Bolscher BG, Hilarius PM, Weening RS, Ochs HD, Roos D, Verhoeven AJ (Mayıs 1994). "A point mutation in gp91-phox of cytochrome b558 of the human NADPH oxidase leading to defective translocation of the cytosolic proteins p47-phox and p67-phox". The Journal of Clinical Investigation. 93 (5): 2120-6. doi:10.1172/JCI117207. PMC 294341 $2. PMID 8182143.
Meindl A, Carvalho MR, Herrmann K, Lorenz B, Achatz H, Lorenz B, Apfelstedt-Sylla E, Wittwer B, Ross M, Meitinger T (Aralık 1995). "A gene (SRPX) encoding a sushi-repeat-containing protein is deleted in patients with X-linked retinitis pigmentosa". Human Molecular Genetics. 4 (12): 2339-46. doi:10.1093/hmg/4.12.2339. PMID 8634708.
Sathyamoorthy M, de Mendez I, Adams AG, Leto TL (Nisan 1997). "p40(phox) down-regulates NADPH oxidase activity through interactions with its SH3 domain". The Journal of Biological Chemistry. 272 (14): 9141-6. doi:10.1074/jbc.272.14.9141. PMID 9083043.
Eklund EA, Kakar R (Nisan 1997). "Identification and characterization of TF1(phox), a DNA-binding protein that increases expression of gp91(phox) in PLB985 myeloid leukemia cells". The Journal of Biological Chemistry. 272 (14): 9344-55. doi:10.1074/jbc.272.14.9344. PMID 9083071.
Jendrossek V, Ritzel A, Neubauer B, Heyden S, Gahr M (Şubat 1997). "An in-frame triplet deletion within the gp91-phox gene in an adult X-linked chronic granulomatous disease patient with residual NADPH-oxidase activity". European Journal of Haematology. 58 (2): 78-85. doi:10.1111/j.1600-0609.1997.tb00928.x. PMID 9111587.
Rae J, Newburger PE, Dinauer MC, Noack D, Hopkins PJ, Kuruto R, Curnutte JT (Haziran 1998). "X-Linked chronic granulomatous disease: mutations in the CYBB gene encoding the gp91-phox component of respiratory-burst oxidase". American Journal of Human Genetics. 62 (6): 1320-31. doi:10.1086/301874. PMC 1377153 $2. PMID 9585602.
Ariga T, Furuta H, Cho K, Sakiyama Y (Temmuz 1998). "Genetic analysis of 13 families with X-linked chronic granulomatous disease reveals a low proportion of sporadic patients and a high proportion of sporadic carriers". Pediatric Research. 44 (1): 85-92. doi:10.1203/00006450-199807000-00014. PMID 9667376.
Kumatori A, Faizunnessa NN, Suzuki S, Moriuchi T, Kurozumi H, Nakamura M (Ekim 1998). "Nonhomologous recombination between the cytochrome b558 heavy chain gene (CYBB) and LINE-1 causes an X-linked chronic granulomatous disease". Genomics. 53 (2): 123-8. doi:10.1006/geno.1998.5510. PMID 9790760.

Dış bağlantılar

Medical Subject Headings gp91phox+protein,+human

[entrez-1] "Entrez Gene: CYBB cytochrome b-245, beta polypeptide (chronic granulomatous disease)". 2 Kasım 2008 tarihinde kaynağından arşivlendi.

[2] "NADPH oxidases in Eukaryotes: red algae provide new hints!". Current Genetics. 49 (3): 190-204. Mart 2006. doi:10.1007/s00294-005-0044-z. PMID 16344959.

[3] "Molecular evolution of Phox-related regulatory subunits for NADPH oxidase enzymes". BMC Evolutionary Biology. 7: 178. Eylül 2007. doi:10.1186/1471-2148-7-178. PMC 2121648 $2. PMID 17900370.

[:0-4] Aguirre (2010). "Nox enzymes from fungus to fly to fish and what they tell us about Nox function in mammals". Free Radical Biology and Medicine. 49 (9): 1342-1353. doi:10.1016/j.freeradbiomed.2010.07.027. PMC 2981133 $2. PMID 20696238.

[one-5] Sorescu (2002). "Superoxide production and expression of nox family proteins in human atherosclerosis". Circulation. 105 (12): 1429-35. doi:10.1161/01.cir.0000012917.74432.66. PMID 11914250.

[two-6] Chaubey (2013). "Nox2 is required for macrophage chemotaxis towards CSF-1". PLOS ONE. 8 (2): e54869. doi:10.1371/journal.pone.0054869. PMC 3562318 $2. PMID 23383302.

[six-7] "NOX2 interacts with podocyte TRPC6 channels and contributes to their activation by diacylglycerol: essential role of podocin in formation of this complex". American Journal of Physiology. Cell Physiology. 305 (9): C960-71. Kasım 2013. doi:10.1152/ajpcell.00191.2013. PMID 23948707.

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