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Asian Journal of Biochemistry

Year: 2022 | Volume: 17 | Issue: 1 | Page No.: 15-24
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Authors

Paul Chijioke Ozioko

Paul Chijioke Ozioko

Yusuf Yunusa Muhammad

Aminu Ibrahim

Aminu Ibrahim

Keywords

Research Article

Ageratum conyzoides Methanol Leaf Extract: Phytochemicals with Antidiabetic Potential via Antioxidant Activity

Paul Chijioke Ozioko
Biology Unit, Faculty of Science, Air Force Institute of Technology, Kaduna, Nigeria
LiveDNA: 234.37555
Yusuf Yunusa Muhammad
Department of Biochemistry, Faculty of Basic Medical Sciences, Bayero University, Kano, Nigeria
Aminu Ibrahim
Department of Biochemistry, Faculty of Basic Medical Sciences, Bayero University, Kano, Nigeria
LiveDNA: 234.20600
Background and Objective: Ageratum conyzoides L. is a traditional herbaceous plant that belongs to the family Asteraceae. The folk and traditional uses of the plant include sleeping sickness, anti-inflammatory, insecticidal, etc. The present study aimed at exploring the possible antidiabetic activity of A. conyzoides through its antioxidant potential in search of new hit compounds. Materials and Methods: The crude extract after maceration was subjected to solvent-solvent fractionation using chloroform, ethyl acetate and ethanol, in increasing order of polarity, followed by quantitative phytochemical estimation. Antioxidant activity was carried out using DPPH radical scavenging assay method. Antidiabetic activities were determined using alloxan-induced diabetic rats, 150 mg of alloxan per kg body weight was given intraperitoneally. All statistical analyses were performed using the SPSS statistical package with data reported as the Mean±SEM. Results: Ethyl acetate fraction had the highest value of TPC (46066.87±1350 mg GAE) and TFC (29912.50±6230 mg QuE) per gram dry samples respectively. Similarly, the ethyl acetate fraction had the best relative antioxidant activity (IC50 of 0.75 μg mL–1). Both crude and the fractions given in different doses exhibited a good blood-glucose-lowering effect with statistically significant difference (p<0.05) between the means of the different groups (and control). The chloroform and ethyl acetate fractions had a better anti-diabetic effect as all administered doses had their average glucose level below the diabetic index (250 mg dL–1). Conclusion: This research showed that A. conyzoides methanol leaf extract has high flavonoid and phenolic contents. It equally demonstrated good antioxidant and antidiabetic activities, especially the ethyl acetate fraction.
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