Abstract
Chromosome aberrations such as loss of chromosome 13 were frequently observed in human endothelial cells from umbilical cord veins (HUVEC). A recent study showed that the length of telomeric single-stranded 3'-overhangs (G-tails) is more important as an essential structure for chromosome maintenance than the net telomere length in telomere t-loop formation. Here, we have examined G-tail length using G-tail telomere HPA in normal and hTERT-transduced HUVECs. We found that forced expression of hTERT in HUVEC induced G-tail as well as total telomere length elongation. G-tail length was well correlated with total telomere length. However, hTERT introduction did not prevent chromosome aberrations such as loss of chromosome 13. Normal characteristics such as morphology, up-regulation of vWF, and tube formation were observed in hTERT-HUVEC as in young normal HUVEC. These results show that chromosome aberrations in HUVEC are independent of telomere G-tail and total telomere attrition.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Blotting, Western
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Cell Proliferation
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Cells, Cultured
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Cellular Senescence / genetics
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Chromosome Aberrations*
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DNA, Single-Stranded / chemistry
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DNA, Single-Stranded / genetics*
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Endothelial Cells / cytology
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Endothelial Cells / metabolism*
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Fibroblast Growth Factor 2 / genetics
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Fibroblast Growth Factor 2 / metabolism
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Gene Expression Profiling
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Gene Expression Regulation, Enzymologic
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Genetic Vectors / genetics
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HeLa Cells
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Humans
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Immunohistochemistry
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Karyotyping
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Microscopy, Fluorescence
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Telomerase / genetics
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Telomerase / metabolism*
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Telomere / genetics*
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Telomere / metabolism
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Transfection
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Vascular Endothelial Growth Factor A / genetics
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Vascular Endothelial Growth Factor A / metabolism
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von Willebrand Factor / genetics
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von Willebrand Factor / metabolism
Substances
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DNA, Single-Stranded
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RNA, Messenger
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Vascular Endothelial Growth Factor A
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von Willebrand Factor
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Fibroblast Growth Factor 2
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Telomerase