Figure 3. Pericyte/SMCs-specific deletion of TrkB induces a reduction in pericyte density.
(A) Double immunofluorescence staining of the left ventricular wall from P21 mice. TrkB (red) is expressed by perivascular cells (PECAM in green) in wild type mice and is largely reduced in trkbf/f-SMCCre+ littermates. (B–G) Quantification of NG2+ (B, C, D) and PECAM+ (E, F, G) cells and intensity in the heart. n = 3 per genotype. Bars represent mean ± s.e.m. Statistical comparisons were made by one-way analysis of variance test. (C) *: P = 0.0023. (D) *: P = 0.007. (F) *: P = 0.0022. Scale bar A,B and E = 20 µm.