Figure 5. Flow cytometry analysis of airway epithelial stem cells.
Flow cytometry analysis using Sftpc and Scgb1a1 (A, C, and E) versus Fgfr2 and Scgb1a1 (B, D, and F) antibodies on permeabilized epithelial cells from WT noninjured lungs (A and B), WT lungs 7 days after naphthalene injury (C and D), and Rosa26-rtTa;Tet-Fgf10 lungs 7 days after naphthalene injury (E and F). A 14-fold increase in double-positive airway epithelial cells was detected with both antibody combinations 7 days after naphthalene injury; a 22- to 33-fold increase was seen 7 days after injury in mice overexpressing Fgf10. Forward scatter/side scatter (FS/SS) plots indicated that the same pool of airway epithelial stem cells was isolated using either antibody combination.