(Q37557649)

21 August 2017

Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli (English)

1 reference

Escherichia coli proteins

21 August 2017

0 references

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1830665

transmembrane transport proteins

4 December 2022

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1830665

4 December 2022

author name string

J. Akimaru

1

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1830665

4 December 2022

S. Matsuyama

2

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1830665

4 December 2022

H. Tokuda

3

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1830665

4 December 2022

S. Mizushima

4

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1830665

4 December 2022

language of work or name

English

0 references

publication date

1 August 1991

1 reference

PMC publication ID

52123

full work available at URL

21 August 2017

https://europepmc.org/articles/PMC52123

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1830665

https://europepmc.org/articles/PMC52123?pdf=render

4 December 2022

file format

Portable Document Format

online access status

open access

content deliverer

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1830665

https://pnas.org/doi/pdf/10.1073/pnas.88.15.6545

4 December 2022

1 reference

Crossref

DOI

10.1073/PNAS.88.15.6545

https://api.crossref.org/works/10.1073/PNAS.88.15.6545

Proceedings of the National Academy of Sciences of the United States of America

4 December 2022

published in

1 reference

21 August 2017

88

1 reference

21 August 2017

15

1 reference

21 August 2017

6545-6549

1 reference

Protein measurement with the Folin phenol reagent

21 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

Phosphorus assay in column chromatography

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

Reconstitution of protein translocation from detergent-solubilized Escherichia coli inverted vesicles: PrlA protein-deficient vesicles efficiently translocate precursor proteins

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

The secD locus of E.coli codes for two membrane proteins required for protein export

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

Solubilization and functional reconstitution of the protein-translocation enzymes of Escherichia coli

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

E. coli mutant pleiotropically defective in the export of secreted proteins

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

In vitro translocation of bacterial secretory proteins and energy requirements

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

SecA protein: autoregulated initiator of secretory precursor protein translocation across the E. coli plasma membrane

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

The SecY membrane component of the bacterial protein export machinery: analysis by new electrophoretic methods for integral membrane proteins

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

Efficient in vitro translocation into Escherichia coli membrane vesicles of a protein carrying an uncleavable signal peptide. Characterization of the translocation process.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

The secE gene encodes an integral membrane protein required for protein export in Escherichia coli.

25 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

In vitro analysis of the process of translocation of OmpA across the Escherichia coli cytoplasmic membrane. A translocation intermediate accumulates transiently in the absence of the proton motive force.

25 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

A high concentration of SecA allows proton motive force-independent translocation of a model secretory protein into Escherichia coli membrane vesicles.

25 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

Reconstitution of translocation activity for secretory proteins from solubilized components of Escherichia coli.

25 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

The binding cascade of SecB to SecA to SecY/E mediates preprotein targeting to the E. coli plasma membrane

25 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation.

25 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

Proton motive force-dependent and -independent protein translocation revealed by an efficient in vitro assay system of Escherichia coli.

25 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=52123

Purification of SecE and reconstitution of SecE-dependent protein translocation activity

1 September 2018

1 reference

https://pubmed.ncbi.nlm.nih.gov/1830665

12 December 2020

In vitro translocation of protein across Escherichia coli membrane vesicles requires both the proton motive force and ATP

1 reference

https://pubmed.ncbi.nlm.nih.gov/1830665

12 December 2020

Mutant isolation and cloning of the gene encoding protease VII from Escherichia coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/1830665

12 December 2020

SecA protein is directly involved in protein secretion in Escherichia coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/1830665

12 December 2020

SecA interacts with secretory proteins by recognizing the positive charge at the amino terminus of the signal peptide in Escherichia coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/1830665

12 December 2020

PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/1830665

12 December 2020

SecE-dependent overproduction of SecY in Escherichia coli. Evidence for interaction between two components of the secretory machinery

1 reference

https://pubmed.ncbi.nlm.nih.gov/1830665

12 December 2020

A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins

1 reference

https://pubmed.ncbi.nlm.nih.gov/1830665

12 December 2020

Identifiers

DOI

10.1073/PNAS.88.15.6545

1 reference

21 August 2017

0 references

1 reference

PMC publication ID

52123

21 August 2017

1 reference

PMC publication ID

52123