Batman
Joker
Bullock
Riddler
Hugo Strange
Mad Hatter
Poison Ivy
Harley Quinn
Catwoman
Mr. Freeze
Zsasz (as Guy Gundy)
Condiment King
Killer Croc (as Lionel Washington)
Barbara Gordon / Oracle
Little Alice
Commissioner James (Jim) Gordon
Summer Gleeson (TV Reporter)
Officer Brooks
Officer Kimble
Officer Kathy (Police Radio)
Arkham Guard
Arkham Doctor