- Famously damaged his knee in 2009 during a dance off with his 500 Days of Summer (2009) co-stars Geoffrey Arend and Joseph Gordon-Levitt in a Mexican dive bar. He couldn't walk for over six months. After a third surgery, Gubler started using a cane but was unable to walk normally for the next five months. As of December 2009, Gubler was once again able to walk unassisted.
- On October 27, 2014, he became a certified Minister. He officiated the marriage of fellow Criminal Minds (2005) star Paget Brewster and best friend Steve Damstra II on November 28, 2014.
- He has also worked as a model for fashion designers as Marc Jacobs, Burberry, Tommy Hilfiger, Sisley, and Louis Vuitton.
- Hosts his own awards ceremony at Criminal Minds (2005) called the "Goobie Awards", where he presents awards to the crew for such categories as Best Facial Hair and Craziest Accent.
- He never wears matching socks, as he says they are bad luck, which he learned from his grandmother. The one time he wore matching socks, he was filming a scene in The Life Aquatic with Steve Zissou (2004). He ended up spraining his ankle on screen, which was kept in the movie.
- His favorite director is Wes Anderson.
- Loves to have things monogrammed.
- Describes himself as a neat freak.
- He is a 2002 graduate of the NYU's Tisch School of the Arts, where he majored in film directing.
- Stated on the season 1 DVD features of Criminal Minds (2005) that his kiss with guest star Amber Heard was his first on-screen kiss.
- Has a brother, Gray Gubler (who appeared in the music video "Reagan by Whirlwind Heat", which Matthew directed).
- He is of Swiss-German, German, English, Irish, Manx, Scottish, and Dutch ancestry.
- His favorite TV show is the HBO series Carnivàle (2003).
- Matthew is an English form of Hebrew name "Matityahu", meaning "Gift of Yahweh".
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