Titel: | Protein gel detection and imaging |
Titelzusatz: | methods and protocols |
Mitwirkende: | Kurien, Biji T. [HerausgeberIn] |
| Scofield, R. Hal [HerausgeberIn] |
Verf.angabe: | edited by Biji T. Kurien, R. Hal Scofield |
Verlagsort: | New York, NY |
Verlag: | Humana Press |
E-Jahr: | 2018 |
Jahr: | [2018] |
Umfang: | xiii, 289 Seiten |
Illustrationen: | Illustrationen |
Gesamttitel/Reihe: | Methods in molecular biology ; 1853 |
| Springer protocols |
Fussnoten: | Includes index |
Ang. zum Inhalt: | Protein stains and applications / Pazhani Sundaram |
| Roles of acetic acid and methanol during fixing and staining proteins in an SDS-polyacrylamide electrophoresis gel / J. P. Dean Goldring |
| Multicolored prestained standard protein marker generation using a variety of remazol dyes for easy visualization of protein bands during SDS-PAGE / Gaurav Kumar |
| Coomassie brilliant blue staining of polyacrylamide gels / Claudia Arndt, Stefanie Koristka, Anja Feldmann, Ralf Bergmann, and Michael Bachmann |
| Simple, time-saving dye staining of proteins in sodium dodecyl sulfate-polyacrylamide gel using coomassie blue / Wei-hua Dong, Fang Wang, Jun-he Zhang, Yan-sheng Zhou, Ling-ye Zhang, and Tian-yun Wang |
| Application of heat to quickly stain and destain proteins stained with coomassie blue / Biji T. Kurien and R. Hal Scofield |
| Silver staining techniques of polyacrylamide gels / Nicole Berndt, Ralf Bergmann, Claudia Arndt, Stefanie Koristka, and Michael Bachmann |
| Counterion dye staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tryptic gel digestion of stained protein for mass spectrometry / Sun-Young Hwang and Jung-Kap Choi |
| Detection of phosphoproteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 8-quinolinol stain / Sun-Young Hwang, Xu Wang, and Jung-Kap Choi |
| Microwave-assisted protein staining, destaining, and in-gel/in-solution digestion of proteins / Jennie R. Lill and Victor J. Nesatyy |
| Fluorescent staining of gels / Engelbert Buxbaum |
| Single-step simultaneous protein staining procedure for polyacrylamide gels and nitrocellulose membranes by alta during western blot analysis / Jayanta K. Pal, Sunil K. Berwal, and Rupali N. Soni |
| TEMED enhanced photoluminescent imaging of human serum proteins by quantum dots after PAGE / Na Na and Jin Ouyang |
| Detection of glycoproteins in polyacrylamide gels using Pro-Q emerald 300 dye, a fluorescent periodate schiff-base stain / Padmaja Mehta-D'souza |
| Curcumin/turmeric as an environment-friendly protein gel stain / Biji T. Kurien, Yaser Dorri, and R. Hal Scofield |
| Detection of multiple enzymes in fermentation broth using single PAGE analysis / K. Divakar, J. Deepa Arul Priya, G. Panneer Selvam, M. Suryia Prabha, Ashwin Kannan, G. Nandhini Devi, and Pennathur Gautam |
| Revisit of imidazole-zinc reverse stain for protein polyacrylamide gel electrophoresis / Han-Min Chen |
| One-step staining protocol for in-gel fluorescent visualization of proteins / Jelena Bogdanović Pristov and Ivan Spasojević |
| Ten minute stain to detect proteins in polyacrylamide electrophoresis gels with direct red 81 and amido black / J. P. Dean Goldring and Robert G. E. Krause |
| In-gel protein phosphatase assay using fluorogenic substrates / Isamu Kameshita, Noriyuki Sueyoshi, and Atsuhiko Ishida |
| Detection of proteins in polyacrylamide gels via prelabeling by isatoic anhydride / Kazem Asadollahi, Saharnaz Rafiee, and Gholamhossein Riazi |
| Fluorescent protein visualization immediately after gel electrophoresis using an in-gel trichloroethanol photoreaction with tryptophan / Carol L. Ladner-Keay, Raymond J. Turner, and Robert A. Edwards |
| Direct immunodetection of antigens within the precast polyacrylamide gel / Surbhi Desai, Boguslawa R. Dworecki, and Marie C. Nlend |
| Zymographic determination of intrinsic specific activity of oxidases in the presence of interfering proteins / Tien Canh Le, Mircea Alexandru Mateescu, Samaneh Ahmadifar, Lucia Marcocci, and Paola Pietrangeli |
| Simple method for detecting phosphorylation of proteins by using Zn2+-Phos-Tag SDS-PAGE at neutral pH / Gaurav Kumar |
| Principle and method of silver staining of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis / Gaurav Kumar |
| Heat/pressure treatment with detergents significantly increases curcumin solubility and stability : its use as an environment-friendly protein gel stain / Biji T. Kurien, Rohit Thomas, Adam Payne, and R. Hal Scofield |
| Fungal laccase efficiently destains coomassie brilliant blue-R-250 stained polyacrylamide gels / Gaurav Kumar |
| Destaining coomassie brilliant blue-stained sodium dodecyl sulfate-polyacrylamide protein gels using a household detergent / Rachna Aggarwal and Biji T. Kurien |
| Paper adsorbents remove coomassie blue from gel destain and used gel stain in an environment-friendly manner / Yaser Dorri and Biji T. Kurien |
| Gel drying methods / Anja Feldmann, Nicole Berndt, Ralf Bergmann, and Michael Bachmann |
| Stained gels can be stored for several months in nonsealed polyethylene bags / Biji T. Kurien and R. Hal Scofield |
| Radiolabeling and analysis of labeled proteins / Nicole Berndt, Ralf Bergmann, and Michael Bachmann. |
ISBN: | 978-1-4939-8744-3 |
| 978-1-4939-8744-3 |
Schlagwörter: | (s)Proteine / (s)Gelelektrophorese |
| (s)Proteine / (s)Gelelektrophorese |
Sprache: | eng |
Bibliogr. Hinweis: | Erscheint auch als : Online-Ausgabe: Protein Gel Detection and Imaging. - New York, NY : Humana Press, 2018. - Online-Ressource (XIII, 289 p. 39 illus., 35 illus. in color, online resource) |
RVK-Notation: | WC 4150 |
| VG 8400 |
Sach-SW: | Gel electrophoresis |
| Proteins |
K10plus-PPN: | 1029966702 |
Verknüpfungen: | → Übergeordnete Aufnahme |
Protein gel detection and imaging / Kurien, Biji T. [HerausgeberIn]; [2018]