| Titel: | Drosophila |
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| Titelzusatz: | methods and protocols |
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| Mitwirkende: | Dahmann, Christian [HerausgeberIn]  |
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| Verf.angabe: | edited by Christian Dahmann |
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| Ausgabe: | Second edition |
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| Verlagsort: | New York |
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| Verlag: | Humana Press |
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| E-Jahr: | 2016 |
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| Jahr: | [2016] |
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| Umfang: | xii, 355 Seiten |
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| Illustrationen: | Illustrationen |
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| Gesamttitel/Reihe: | Methods in molecular biology ; 1478 |
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| | Springer protocols |
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| Fussnoten: | Includes bibliographical references and index |
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| Ang. zum Inhalt: | Using FlyBase, a database of Drosophila genes and genomes / Steven J. Marygold [and others] |
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| | Using FlyBase, a database of Drosophila genes and genomes / Steven J. Marygold ... [et al.] |
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| | GAL4 system : a versatile system for the manipulation and analysis of gene expression / Elizabeth E. Caygill and Andrea H. Brand |
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| | Q-System : a versatile expression system for Drosophila / Olena Riabinina and Christopher J. Potter |
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| | Analysis of microRNA runction in Drosophila / Geetanjali Chawla, Arthur Luhur, and Nicholas Sokol |
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| | Methods for high-throughput RNAi screening in Drosophila cells / Maximilian Billmann and Michael Boutros |
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| | Guide to genome-wide in vivo RNAi applications in Drosophila / Aynur Kaya-Çopur and Frank Schnorrer |
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| | Creating heritable mutations in Drosophila with CRISPR-Cas9 / Fillip Port and Simon L. Bullock |
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| | Performing chromophore-assisted laser inactivation in Drosophila embryos using GFP / Anne Pélissier-Monier, Bénédicte Sanson, and Bruno Monier |
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| | deGradFP : a system to knockdown GFP-tagged proteins / Emmanuel Caussinus and Markus Affolter |
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| | Sample preparation and mounting of Drosophila embryos for multiview light sheet microscopy / Christopher Schmied and Pavel Tomancak |
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| | Cultivation and live imaging of Drosophila imaginal discs / Simon Restrepo, Jeremiah J. Zartman, and Konrad Basler |
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| | Cultivation and live imaging of Drosophila ovaries / Maureen Cetera, Lindsay Lewellyn, and Sally Horne-Badovinac |
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| | Segmentation and quantitative analysis of epithelial tissues / Benoit Aigouy, Daiki Umetsu, and Suzanne Eaton |
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| | Laser ablation to probe the epithelial mechanics in Drosophila / Pruthvi C. Shivakumar and Pierre-Francois Lenne |
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| | Rapid ovary mass-isolation (ROMi) to obtain large quantities of Drosophila egg chambers for fluorescent in situ hybridization / Helena Jambor, Pavel Mejstrik, and Pavel Tomancak |
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| | Chromatin immunoprecipitation for analyzing transcription factor binding and histone modifications in Drosophila / Yad Ghavi-Helm, Bingqing Zhao, and Eileen E.M. Furlong |
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| | Protocols to study growth and metabolism in Drosophila / Katrin Strassburger and Aurelio A. Teleman |
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| | Protocols to study aging in Drosophila / Matthew D.W. Piper and Linda Partridge |
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| | Protocols to study behavior in Drosophila / Wendi S. Neckameyer and Parag Bhatt |
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| | Electrophysiological recordings from lobula plate tangential cells in Drosophila / Alex S. Mauss and Alexander Borst |
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| | Methods to establish Drosophila cell lines / Alain Debec, Timothy L. Megraw, and Antoine Guichet. |
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| | GAL4 system : a versatile system for the manipulation and analysis of gene expression / Elizabeth E. Caygill and Andrea H. Brand |
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| | Q-System : a versatile expression system for Drosophila / Olena Riabinina and Christopher J. Potter |
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| | Analysis of microRNA runction in Drosophila / Geetanjali Chawla, Arthur Luhur, and Nicholas Sokol |
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| | Methods for high-throughput RNAi screening in Drosophila cells / Maximilian Billmann and Michael Boutros |
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| | Guide to genome-wide in vivo RNAi applications in Drosophila / Aynur Kaya-Çopur and Frank Schnorrer Creating heritable mutations in Drosophila with CRISPR-Cas9 |
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| | Performing chromophore-assisted laser inactivation in Drosophila embryos using GFP / Anne Pélissier-Monier, Bénédicte Sanson, and Bruno Monier |
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| | deGradFP : a system to knockdown GFP-tagged proteins / Emmanuel Caussinus and Markus Affolter |
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| | Sample preparation and mounting of Drosophila embryos for multiview light sheet microscopy / Christopher Schmied and Pavel Tomancak |
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| | Cultivation and live imaging of Drosophila imaginal discs / Simon Restrepo, Jeremiah J. Zartman, and Konrad Basler |
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| | Cultivation and live imaging of Drosophila ovaries / Maureen Cetera, Lindsay Lewellyn, and Sally Horne-Badovinac |
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| | Segmentation and quantitative analysis of epithelial tissues / Benoit Aigouy, Daiki Umetsu, and Suzanne Eaton |
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| | Laser ablation to probe the epithelial mechanics in Drosophila / Pruthvi C. Shivakumar and Pierre-Francois Lenne |
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| | Rapid ovary mass-isolation (ROMi) to obtain large quantities of Drosophila egg chambers for fluorescent in situ hybridization / Helena Jambor, Pavel Mejstrik, and Pavel Tomancak |
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| | Chromatin immunoprecipitation for analyzing transcription factor binding and histone modifications in Drosophila / Yad Ghavi-Helm, Bingqing Zhao, and Eileen E. M. Furlong |
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| | Protocols to study growth and metabolism in Drosophila / Katrin Strassburger and Aurelio A. Teleman |
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| | Protocols to study aging in Drosophila / Matthew D. W. Piper and Linda Partridge |
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| | Protocols to study behavior in Drosophila / Wendi S. Neckameyer and Parag Bhatt |
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| | Electrophysiological recordings from lobula plate tangential cells in Drosophila / Alex S. Mauss and Alexander Borst |
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| | Methods to establish Drosophila cell lines / Alain Debec, Timothy L. Megraw, and Antoine Guichet. |
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| ISBN: | 978-1-4939-6369-0 |
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| Abstract: | "This second edition volume expands on the previous edition by presenting updated protocols for several of the techniques described in the first edition of Drosophila: Methods and Protocols and current methods that cover recent breakthroughs in Drosophila research. The book begins with a description of FlyBase--a database of genes and genomes--followed by the presentation of systems for versatile gene expression in the fly. The first few chapters in this book detail gene knockdown and editing, including CRISPR-Cas9 and protein knockdown. The next few chapters are devoted to methods describing live imaging of different tissues and organs, followed by chapters on how to quantify image data and how to probe tissue mechanics by laser ablation. The next two chapters provide methods for analyzing transcription followed by protocols to study growth, metabolism, ageing, and behavior in Drosophila. This volume concludes with chapters on electrophysiological recordings and methods to establish cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls"-- |
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| URL: | Verlag: https://link.springer.com/book/10.1007/978-1-4939-6371-3 |
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| Schlagwörter: | (s)Drosophila |
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| Sprache: | eng |
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| Bibliogr. Hinweis: | Erscheint auch als : Online-Ausgabe: Drosophila. - 2nd ed. 2016. - New York, NY : Humana Press, 2016. - Online-Ressource (XII, 355 p. 72 illus., 41 illus. in color, online resource) |
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| RVK-Notation: | WC 6340 |
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| | WC 4460 |
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| | WC 4150 |
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| K10plus-PPN: | 871400545 |
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| Verknüpfungen: | → Übergeordnete Aufnahme |
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