Titel:	The Wilms' tumor (WT1) gene
Titelzusatz:	methods and protocols
Mitwirkende:	Hastie, Nicholas [HerausgeberIn]
Verf.angabe:	edited by Nicholas Hastie (MRC Human Genetics Unit, University of Edinburgh, Edinburgh, UK)
Verlagsort:	New York
Verlag:	Humana Press, Springer Science+Business Media
E-Jahr:	2016
Jahr:	[2016]
Umfang:	x, 235 Seiten
Illustrationen:	Illustrationen
Format:	26 cm
Gesamttitel/Reihe:	Methods in molecular biology ; 1467
	Springer protocols
Ang. zum Inhalt:	WT1 mutation in childhood cancer / Jocelyn Charlton and Kathy Pritchard-Jones
	WT1 mutation in childhood cancer / Jocelyn Charlton and Kathy Pritchard-Jones
	Clinical aspects of WT1 and the kidney / Eve Miller-Hodges
	Role of WT1 in embryonic development and normal organ homeostasis / Bettina Wilm and Ramon Muñoz-Chapuli
	Tools and techniques for Wt1-based lineage tracing / Bettina Wilm and Ramon Muñoz-Chapuli
	Biological systems and methods for studying WT1 in the epicardium / Víctor Velecela, Janat Fazal-Salom, and Ofelia M. Martínez-Estrada
	Isolation and colony formation of murine bone and bone marrow cells / Sophie McHaffie and You-Ying Chau
	Isolation and fluorescence-activated cell sorting of murine WT1-expressing adipocyte precursor cells / Louise Cleal and You-Ying Chau
	In vivo assays for assessing the role of the wilms' tumor suppressor 1 (Wt1) in angiogenesis / Richard J. McGregor [and others]
	Multiphoton microscopy for visualizing lipids in tissue / Martin Lee and Alan Serrels
	Function and regulation of the wilms tumor suppressor 1 (WT1) gene in fish / Birgit Perner [and others]
	Immunofluorescence staining of WT1 on sections of zebrafish embryos and larvae / Birgit Perner and Christoph Englert
	Fluorescence-activated cell sorting (FACS) protocol for podocyte isolation in adult zebrafish / Thomas J.D. Bates, Uta Naumann, and Christoph Englert
	In vitro transcription to study WT1 function / Stefan G.E. Roberts
	Measuring equilibrium binding constants for the WT1-DNA interaction using a filter binding assay / Paul J. Romaniuk
	Identifying direct downstream targets : WT1 ChIP-Seq analysis / Fabio da Silva, Filippo Massa, and Andreas Schedl
	WT1 associated protein-protein interaction networks / Ruthrothaselvi Bharathavikru and Alex von Kriegsheim
	Methods to identify and validate WT1-RNA interaction / Ruthrothaselvi Bharathavikru and Tatiana Dudnakova
	Bioinformatic analysis of next-generation sequencing data to identify WT1-associated differential gene and isoform expression / Stuart Aitken and Ruthrothaselvi Bharathavikru
	Immunotherapy targeting WT1 : designing a protocol for WT1 peptide-based cancer vaccine / Sumiyuki Nishida and Haruo Sugiyama.
	Clinical aspects of WT1 and the kidney / Eve Miller-Hodges
	Role of WT1 in embryonic development and normal organ homeostasis / Bettina Wilm and Ramon Muñoz-Chapuli
	Tools and techniques for Wt1-based lineage tracing / Bettina Wilm and Ramon Muñoz-Chapuli
	Biological systems and methods for studying WT1 in the epicardium / Víctor Velecela, Janat Fazal-Salom, and Ofelia M. Martínez-Estrada
	Isolation and colony formation of murine bone and bone marrow cells / Sophie McHaffie and You-Ying Chau
	Isolation and fluorescence-activated cell sorting of murine WT1-expressing adipocyte precursor cells / Louise Cleal and You-Ying Chau
	In vivo assays for assessing the role of the wilms' tumor suppressor 1 (Wt1) in angiogenesis / Richard J. McGregor ... [et al.]
	Multiphoton microscopy for visualizing lipids in tissue / Martin Lee and Alan Serrels
	Function and regulation of the wilms tumor suppressor 1 (WT1) gene in fish / Birgit Perner ... [et al.]
	Immunofluorescence staining of WT1 on sections of zebrafish embryos and larvae / Birgit Perner and Christoph Englert
	Fluorescence-activated cell sorting (FACS) protocol for podocyte isolation in adult zebrafish / Thomas J. D. Bates, Uta Naumann, and Christoph Englert
	In vitro transcription to study WT1 function / Stefan G. E. Roberts
	Measuring equilibrium binding constants for the WT1-DNA interaction using a filter binding assay / Paul J. Romaniuk
	Identifying direct downstream targets : WT1 ChIP-Seq analysis / Fabio da Silva, Filippo Massa, and Andreas Schedl
	WT1 associated protein-protein interaction networks / Ruthrothaselvi Bharathavikru and Alex von Kriegsheim
	Methods to identify and validate WT1-RNA interaction / Ruthrothaselvi Bharathavikru and Tatiana Dudnakova
	Bioinformatic analysis of next-generation sequencing data to identify WT1-associated differential gene and isoform expression / Stuart Aitken and Ruthrothaselvi Bharathavikru
	Immunotherapy targeting WT1 : designing a protocol for WT1 peptide-based cancer vaccine / Sumiyuki Nishida and Haruo Sugiyama.
ISBN:	978-1-4939-4021-9
Abstract:	This volume provides a thorough overview of the Wilms Tumour Gene (WT1). The book begins with three review chapters that cover the involvement of WT1 in pediatric cancer, kidney disease, and tissue development and homeostasis. The next few chapters discuss cell marking and lineage tracing, epicardial cell methodology, colony forming assays for bone marrow stem cells, angiogenesis assays and zebrafish tools. The next group of chapters explores the latest tools in genomics, molecular biology, and biochemistry. They discuss dissecting transcription factor function in cell free systems, ChiP seq, proteomics, RNA interactome, and multiphoton imaging of lipids, measuring the binding constants of protein-nucleic acid interactions, and bioinformatics approaches for analyzing Next Generation Sequence data. The final chapter discusses protocols for clinical trials for immune therapy using anti-WT1 peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, The Wilms Tumour (WT1) Gene: Methods and Protocols is a valuable resource for anyone who is interested in the diverse methodologies used in WT1 research
URL:	Cover: http://swbplus.bsz-bw.de/bsz476314682cov.htm
Schlagwörter:	(s)Nephroblastom    / (s)Genetik
	(s)Nephroblastom    / (s)Genetik
Sprache:	eng
Bibliogr. Hinweis:	Erscheint auch als : Online-Ausgabe |(DE-576)476154324
	Erscheint auch als : Online-Ausgabe: The Wilms' Tumor (WT1) Gene. - New York, NY : Humana Press, 2016. - Online-Ressource (X, 235 p. 42 illus., 34 illus. in color, online resource)
RVK-Notation:	XH 5400
K10plus-PPN:	866101705
Verknüpfungen:	→ Übergeordnete Aufnahme