Titel: | The nuclear envelope |
Titelzusatz: | methods and protocols |
Mitwirkende: | Shackleton, Sue [HerausgeberIn] |
| Collas, Philippe [HerausgeberIn] |
| Schirmer, Eric C. [HerausgeberIn] |
Verf.angabe: | Sue Shackleton, Philippe Collas, Eric C. Schirmer |
Verlagsort: | New York |
Verlag: | Humana Press |
E-Jahr: | 2016 |
Jahr: | [2016] |
Umfang: | xiv, 523 Seiten |
Illustrationen: | Illustrationen |
Gesamttitel/Reihe: | Methods in molecular biology ; 1411 |
| Springer protocols |
Ang. zum Inhalt: | Isolation, proteomic analysis, and microscopy confirmation of the liver nuclear envelope proteome / Nadia Korfali, Laurence Florens, and Eric C. Schirmer |
| Exploring the protein composition of the plant nuclear envelope / Xiao Zhou ... [et al.] |
| High-efficiency isolation of nuclear envelope protein complexes from trypanosomes / Samson O. Obado ... [et al.] |
| Superresolution microscopy of the nuclear envelope and associated proteins / Wei Xie, Henning F. Horn, and Graham D. Wright |
| Analyses of the dynamic properties of nuclear lamins by fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) / Shimi Takeshi, Chan-Gi Pack, and Robert D. Goldman |
| Probing protein distribution along the nuclear envelope in vivo by using single-point FRAP / Krishna C. Mudumbi and Weidong Yang |
| Use of two-photon FRET-FLIM to study protein interactions during nuclear envelope fusion in vivo and in vitro / Richard D. Byrne, Banafshé Larijani, and Dominic L. Poccia |
| Identifying protein-protein associations at the nuclear envelope with BioID / Dae In Kim, Samuel C. Jensen, and Kyle J. Roux |
| In situ detection of interactions between nuclear envelope proteins and partners / Alice Barateau and Brigitte Buendia |
| Methods for single-cell pulse-chase analysis of nuclear components / Marek Drozdz and David J. Vaux |
| Analysis of nuclear lamina proteins in myoblast differentiation by functional complementation / Olga Tapia and Larry Gerace |
| Analysis of meiotic telomere behavior in the mouse / Jana Link, Ricardo Benavente, and Manfred Alsheimer |
| Identification and validation of putative nesprin variants / Flavia Autore, Catherine M. Shanahan, and Qiuping Zhang |
| Detection of diverse and high molecular weight nesprin-1 and nesprin-2 isoforms using western blotting / James Carthew and Iakowos Karakesisoglou |
| Use of polyacrylamide hydrogels to study the effects of matrix stiffness on nuclear envelope properties / Rose-Marie Minaisah, Susan Cox, and Derek T. Warren |
| Cell microharpooning to study nucleo-cytoskeletal coupling / Gregory Fedorchak and Jan Lammerding |
| Wound-healing assays to study mechanisms of nuclear movement in fibroblasts and myoblasts / Wakam Chang, Susumu Antoku, and Gregg G. Gundersen |
| Methods for assessing nuclear rotation and nuclear positioning in developing skeletal muscle cells / Meredith H. Wilson, Matthew G. Bray, and Erika L. F. Holzbaur |
| Imaging approaches to investigate myonuclear positioning in Drosophila / Mafalda Azevedo ... [et al.] |
| Mapping nuclear lamin-genome interactions by chromatin immunoprecipitation of nuclear lamins / Anja R. Oldenburg and Philippe Collas |
| Lamin ChIP from chromatin prepared by micrococcal nuclease digestion / Isabelle Duband-Goulet |
| DamID analysis of nuclear organization in Caenorhabditis elegans / Georgina Gómez-Saldivar, Peter Meister, and Peter Askjaer |
| Application of DamID to identify peripheral gene sequences in differentiated and primary cells / Michael I. Robson and Eric C. Schirmer |
| Visualizing the spatial relationship of the genome with the nuclear envelope using fluorescence in situ hybridization / Craig S. Clements ... [et al.] |
| Visualization of genomic loci in living cells with a fluorescent CRISPR/Cas9 system / Tobias Anton, Heinrich Leonhardt, and Yolanda Markaki |
| Methods to monitor DNA repair defects and genomic instability in the context of a disrupted nuclear lamina / Susana Gonzalo and Ray Kreienkamp |
| High-resolution scanning electron microscopy and immuno-gold labeling of the nuclear lamina and nuclear pore complex / Martin W. Goldberg |
| In vitro assay to study targeting of membrane proteins to the inner nuclear membrane / Rosemarie Ungricht, Sumit Pawar, and Ulrike Kutay |
| Nuclear protein transport in digitonin permeabilized cells / Stephen A. Adam |
| Analysis of CRM1-dependent nuclear export in permeabilized cells / Ralph H. Kehlenbach and Sarah A. Port |
| SPEED microscopy and its application in nucleocytoplasmic transport / Jiong Ma, Joseph M. Kelich, and Weidong Yang. |
ISBN: | 978-1-4939-3528-4 |
URL: | Cover: https://swbplus.bsz-bw.de/bsz472890867cov.jpg |
Schlagwörter: | (s)Zellwand |
Sprache: | eng |
Bibliogr. Hinweis: | Erscheint auch als : Online-Ausgabe: The Nuclear Envelope. - New York, NY : Humana Press, 2016. - Online-Ressource (XIV, 523 p. 103 illus., 77 illus. in color, online resource) |
RVK-Notation: | WE 4000 |
K10plus-PPN: | 858701367 |
Verknüpfungen: | → Übergeordnete Aufnahme |