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| Verfasst von: | Guerra, Matteo [VerfasserIn]  |
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| | Frey, Dario [VerfasserIn] |
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| | Hagner, Matthias [VerfasserIn] |
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| | Dittrich, A. Susanne [VerfasserIn] |
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| | Paulsen, Michelle [VerfasserIn] |
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| | Mall, Marcus A. [VerfasserIn] |
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| | Schultz, Carsten [VerfasserIn] |
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| Titel: | Cathepsin G activity as a new marker for detecting airway inflammation by microscopy and flow cytometry |
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| Verf.angabe: | Matteo Guerra, Dario Frey, Matthias Hagner, Susanne Dittrich, Michelle Paulsen, Marcus A. Mall and Carsten Schultz |
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| E-Jahr: | 2019 |
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| Jahr: | February 19, 2019 |
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| Umfang: | 10 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 27.05.2019 |
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| Titel Quelle: | Enthalten in: American Chemical SocietyACS central science |
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| Ort Quelle: | Washington, DC : ACS Publ., 2015 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 5(2019), 3, Seite 539-548 |
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| ISSN Quelle: | 2374-7951 |
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| Abstract: | Muco-obstructive lung diseases feature extensive bronchiectasis due to the uncontrolled release of neutrophil serine proteases into the airways. To assess if cathepsin G (CG) is a novel key player in chronic lung inflammation, we developed membrane-bound (mSAM) and soluble (sSAM) FRET reporters. The probes quantitatively revealed elevated CG activity in samples from 46 patients. For future basic science and personalized clinical applications, we developed a rapid, highly informative, and easily translatable small-molecule FRET flow cytometry assay for monitoring protease activity including cathepsin G. We demonstrated that mSAM distinguished healthy from patient cells by FRET-based flow cytometry with excellent correlation to confocal microscopy data. |
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| DOI: | doi:10.1021/acscentsci.8b00933 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1021/acscentsci.8b00933 |
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| | Volltext: https://doi.org/10.1021/acscentsci.8b00933 |
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| | DOI: https://doi.org/10.1021/acscentsci.8b00933 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1666311308 |
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| Verknüpfungen: | → Zeitschrift |
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Cathepsin G activity as a new marker for detecting airway inflammation by microscopy and flow cytometry / Guerra, Matteo [VerfasserIn]; February 19, 2019 (Online-Ressource)
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