Titel:	Epigenome editing
Titelzusatz:	methods and protocols
Mitwirkende:	Jeltsch, Albert [HerausgeberIn]
	Rots, Marianne G. [HerausgeberIn]
Verf.angabe:	edited by Albert Jeltsch, Marianne G. Rots
Verlagsort:	New York, NY
Verlag:	Humana Press
E-Jahr:	2018
Jahr:	[2018]
Umfang:	xiv, 482 Seiten
Illustrationen:	Illustrationen
Format:	25.4 cm x 17.8 cm
Gesamttitel/Reihe:	Methods in molecular biology ; 1767
	Springer protocols
Fussnoten:	Includes bibliographical references and index
Ang. zum Inhalt:	Editing the epigenome : overview, open questions, and directions of future development / Marianne G. Rots and Albert Jeltsch Zinc fingers, TALEs, and CRISPR systems : a comparison of tools for epigenome editing
	Designing epigenome editors : considerations of biochemical and locus specificities / Dilara Sen and Albert J. Keung
	Generation of TALE-based designer epigenome modifiers / Sandra Nitsch and Claudio Mussolino
	Neuroepigenetic editing / Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, and Elizabeth A. Heller
	Allele-specific epigenome editing / Pavel Bashtrykov and Albert Jeltsch
	Key to delivery : the (Epi-)genome editing vector toolbox / Sabrina Just and Hildegard Büning
	CRISPR/dCas9 switch systems for temporal transcriptional control / Rutger A. F. Gjaltema and Edda G. Schulz
	Delivery of designer epigenome modifiers into primary human T cells / Tafadzwa Mlambo, Marianna Romito, Tatjana I. Cornu, and Claudio Mussolino
	Viral expression of epigenome editing tools in rodent brain using stereotaxic surgery techniques / Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, and Elizabeth A. Heller
	Stable expression of epigenome editors via viral delivery and genomic integration / Carolin Kroll and Philipp Rathert
	Purified protein delivery to activate an epigenetically silenced allele in mouse brain / Benjamin Pyles, Barbara J. Bailus, Henriette O'Geen, and David J. Segal
	Non-viral methodology for efficient co-transfection / Jessica A. Kretzmann, Cameron W. Evans, Marck Norret, Pilar Blancafort, and K. Swaminathan Iyer
	Chromatin immunoprecipitation in human and yeast cells / Jessica B. Lee and Albert J. Keung
	Chromatin immunoprecipitation and high-throughput sequencing (ChIP-Seq) : tips and tricks regarding the laboratory protocol and initial downstream data analysis / Darren K. Patten, Giacomo Corleone, and Luca Magnani
	Generation of whole genome bisulfite sequencing libraries for comprehensive DNA methylome analysis / Dulce B. Vargas-Landin, Jahnvi Pflüger, and Ryan Lister
	Approaches for the analysis and interpretation of whole genome bisulfite sequencing data / Tim Stuart, Sam Buckberry, and Ryan Lister
	Whole-genome bisulfite sequencing for the analysis of genome-wide DNA methylation and hydroxymethylation patterns at single-nucleotide resolution / Magali Kernaleguen, Christian Daviaud, Yimin Shen, Eric Bonnet, Victor Renault, Jean-François Deleuze, Florence Mauger, and Jörg Tost
	Locus-specific DNA methylation analysis by targeted deep bisulfite sequencing / Elsa Leitão, Jasmin Beygo, Michael Zeschnigk, Ludger Klein-Hitpass, Marcel Bargull, Sven Rahmann, and Bernhard Horsthemke
	DNA methylation analysis by bisulfite conversion coupled to double multiplexed amplicon-based next-generation sequencing (NGS) / Pavel Bashtrykov and Albert Jeltsch
	Cell-to-cell transcription variability as measured by single-molecule RNA FISH to detect epigenetic state switching / William Beckman, Ilona M. Vuist, Hermannus Kempe, and Pernette J. Verschure
	Establishment of cell lines stably expressing dCas9-fusions to address kinetics of epigenetic editing / Désirée Goubert, Mihály Koncz, Antal Kiss, and Marianne G. Rots
	Editing of DNA methylation using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions / Sumiyo Morita, Takuro Horii, and Izuho Hatada
	Chemical inducible dCas9-guided editing of H3K27 acetylation in mammalian Cclls / Dan Gao and Fu-Sen Liang
	Screening regulatory element function with CRISPR/Cas9-based epigenome editing / Tyler S. Klann, Gregory E. Crawford, Timothy E. Reddy, and Charles A. Gersbach.
ISBN:	1-4939-7773-3
	978-1-4939-7773-4
Bestellnr.:	978-1-4939-7773-4
	86873331
Abstract:	This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epigenome Editing: Methods and Protocols will be of great assistance to people new to the field but also to those already engaged, as epigenetic editing is still a relatively unexplored field with many issues to be resolved
URL:	Inhaltstext: http://deposit.dnb.de/cgi-bin/dokserv?id=86b20d7f99e342e499883c2f5eeb3e4e&prov=M&dok_var=1&dok_ext=htm
Schlagwörter:	(s)Genom    / (s)Epigenetik    / (s)Methode
	(s)Epigenetik
Sprache:	eng
Reproduktion:	Elektronische Reproduktion
RVK-Notation:	WC 4460
Sach-SW:	Biomedicine
	Human genetics
	Medicine
K10plus-PPN:	1018342699
Verknüpfungen:	→ Übergeordnete Aufnahme