Rubber tree (Hevea brasiliensis) is one of important industrial plants in Indonesia. It is planted widely in some provinces in Indonesia, one of which is in West Java under management of the PTPN VIII plantation, Subang Regency. Intercropping system using underutilized crops Canna sp. (Ganyong) had been introduced on Rubber plantation. The aim of this research is to analyze the diversity, abundance and richness of rhizosphere soil microbial under rubber-canna agroforestry system using metagenomic analysis of 16S rRNA gene of soil rhizosphere bacteria. The research was carried out by collecting soil samples from 2 different soil conditions (soils with Canna (G) and soils without Canna (TG)). 2 different soil depths conditions (20 and 40 cm) were also investigated. The results showed that number of microbial in G soils was found more abundance than in TG soils, while based on soil depth there was no significant effect on soil microbial abundance. Based on class level was found 84 Classes. There were 3 classes that are mostly found in G and TG, namely Ktedonobacteria, Acidobacteria and Planctomycetia. Microbial diversity in family level mostly found in G and TG, Namely Koribacteraceae, Gemmataceae, Synobacteraceae, Hyphomicrobiaceae.