Polymerase chain reaction

Polymerase Chain Reaction (PCR)- Principle, Steps, Applications.

The polymerase chain reaction (PCR) is a laboratory technique for DNA replication that allows a "target" DNA sequence to be selectively amplified. PCR can use

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POLYMERASE CHAIN REACTION (PCR) The polymerase chain reaction (PCR) provides a simple and ingeneous method for exponentially amplification of specific DNA sequences by in vitro DNA synthesis. This technique was developed by Katy Mullis at Cetus Corporation in Emery Ville, California in 1985. Katy Mullis shared the Nobel prize for chemistry in 1993. This technique has made it possible to synthesize large quantities of a DNA fragment without its cloning. It is ideally suited where the quantity…