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the difference between spliced pangenome graphs and pantranscriptomes #4814

@jwli-code

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@jwli-code

I don't understand the difference between spliced pangenome graphs and pantranscriptomes. Aren't the commands the same? https://github.com/vgteam/vg/wiki/Transcriptomic-analyses
I used MC's workflow to construct a graph genome, and then I found that the construction methods for Spliced pangenome graphs and Pantranscriptomes are exactly the same. I want to do the subsequent VPRG analysis. But I found that the command for Spliced pangenome graphs is

vg rna -p --threads <threads> --transcripts annotation.[gtf|gff3] --use-hap-ref --gbz-format graph.gbz > spliced_graph.pg
The command to construct transcriptomic analysis is
vg rna -p --threads <threads> --transcripts annotation.[gtf|gff3] --use-hap-ref --gbz-format --write-gbwt pantranscriptome.gbwt --write-info pantranscriptome.txt graph.gbz > spliced_graph.pg
It seems like the difference is only between these two parameters --write-gbwt pantranscriptome.gbwt --write-info pantranscriptome.txt , but these two parameters are just one output, which I don't quite understand. Maybe there is an issue with the input of the transcript file. I used the genome I constructed and provided all the GFF information for each genome.

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