MMP9
Matrix metalloproteinase-9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is a matrixin, a class of enzymes that belong to the zinc-metalloproteinases family involved in the degradation of the extracellular matrix. In humans the MMP9 gene[5] encodes for a signal peptide, a propeptide, a catalytic domain with inserted three repeats of fibronectin type II domain followed by a C-terminal hemopexin-like domain.[6]
Function
[edit]Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, angiogenesis, bone development, wound healing, cell migration, learning and memory, as well as in pathological processes, such as arthritis, intracerebral hemorrhage,[7] and metastasis.[8] Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens and other extracellular matrix proteins.[9] Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.[5]
Thrombospondins, intervertebral disc proteins, regulate interaction with matrix metalloproteinases (MMPs) 2 and 9, which are key effectors of ECM remodeling.[10]
Neutrophil action
[edit]MMP9, along with elastase, appears to be a regulatory factor in neutrophil migration across the basement membrane.[11]
MMP9 plays several important functions within neutrophil action, such as degrading extracellular matrix, activation of IL-1β, and cleavage of several chemokines.[12] In a mouse model, MMP9 deficiency resulted in resistance to endotoxin shock, suggesting that MMP9 is important in sepsis.[13]
Angiogenesis
[edit]MMP9 may play an important role in angiogenesis and neovascularization. For example, MMP9 appears to be involved in the remodeling associated with malignant glioma neovascularization.[14] It is also a key regulator of growth plate formation- both growth plate angiogenesis and the generation of hypertrophic chondrocytes. Knock-out models of MMP9 result in delayed apoptosis, vascularization, and ossification of hypertrophic chondrocytes.[15] Lastly, there is significant evidence that Gelatinase B is required for the recruitment of endothelial stem cells, a critical component of angiogenesis [16]
Wound repair
[edit]MMP9 is greatly upregulated during human respiratory epithelial healing.[17] Using a MMP9 deficient mouse model, it was seen that MMP9 coordinated epithelial wound repair and deficient mice were unable to remove the fibrinogen matrix during wound healing.[18] When interacting with TGF-ß1, Gelatinase B also stimulates collagen contraction, aiding in wound closure.[19]
Structure
[edit]![](http://upload.wikimedia.org/wikipedia/commons/thumb/0/06/ProMMP9.png/300px-ProMMP9.png)
MMP9 is synthesized as preproenzyme of 707 amino-acid residues, including a 19 amino acid signal peptide and secreted as an inactive pro-MMP. The human MMP9 proenzyme consists of five domains. The amino-terminal propeptide, the zinc-binding catalytic domain and the carboxyl-terminal hemopexin-like domain are conserved. Its primary structure comprises several domain motifs. The propeptide domain is characterized by a conserved PRCGVPD sequence. The Cys within this sequence is known as the “cysteine switch”. It ligates the catalytic zinc to maintain the enzyme in an inactive state.[6]
![](http://upload.wikimedia.org/wikipedia/commons/thumb/d/d2/MMP9-probe_4JIJ.png/400px-MMP9-probe_4JIJ.png)
Activation is achieved through an interacting protease cascade involving plasmin and stromelysin 1 (MMP-3). Plasmin generates active MMP-3 from its zymogen. Active MMP-3 cleaves the propeptide from the 92-kDa pro-MMP-9, yielding an 82-kDa enzymatically active enzyme.[21] In the active enzyme a substrate, or a fluorogenic activity probe.,[20] replaces the propeptide in the enzyme active site where it is cleaved. The catalytic domain contains two zinc and three calcium atoms. The catalytic zinc is coordinated by three histidines from the conserved HEXXHXXGXXH binding motif. The other zinc atom and the three calcium atoms are structural. A conserved methionine, which forms a unique “Met-turn” structure categorizes MMP9 as a metzincin.[22] Three type II fibronectin repeats are inserted in the catalytic domain, although these domains are omitted in most crystallographic structures of MMP9 in complex with inhibitors. The active form of MMP9 also contains a C-terminal hemopexin-like domain. This domain is ellipsoidal in shape, formed by four β-propeller blades and an α-helix. Each blade consists of four antiparallel β-strands arranged around a funnel-like tunnel that contains two calcium and two chloride ions.[23] The hemopexin domain is important to facilitate the cleavage of triple helical interstitial collagens. .
Clinical significance
[edit]MMP9 has been found to be associated with numerous pathological processes, including cancer, placental malaria, immunologic and cardiovascular diseases.
Arthritis
[edit]Elevated MMP9 levels can be found in the cases of rheumatoid arthritis[24] and focal brain ischemia.[25]
Cancer
[edit]One of MMP9's most widely associated pathologies is the relationship to cancer, due to its role in extracellular matrix remodeling and angiogenesis. For example, its increased expression was seen in a metastatic mammary cancer cell line.[26] Gelatinase B plays a central role in tumor progression, from angiogenesis, to stromal remodeling, and ultimately metastasis.[27] However, because of its physiologic function, it may be difficult to leverage Gelatinase B inhibition into cancer therapy modalities. However, Gelatinase B has been investigated in tumor metastasis diagnosis- Complexes of Gelatinase B/Tissue Inhibitors of Metalloproteinases are seen to be increased in gastrointestinal cancer and gynecologic malignancies [28]
MMPs such as MMP9 can be involved in the development of several human malignancies, as degradation of collagen IV in basement membrane and extracellular matrix facilitates tumor progression, including invasion, metastasis, growth and angiogenesis.[29]
Cardiovascular
[edit]MMP9 levels increase with the progression of idiopathic atrial fibrillation.[30]
MMP9 has been found to be associated with the development of aortic aneurysms,[31] and its disruption prevents the development of aortic aneurysms.[32] Doxycycline suppresses the growth of aortic aneurysms in animal models through its inhibition of MMP9 reduces aortic inflammation in humans.[33]
Pregnancy-associated malaria (Placental malaria)
[edit]A study on Ghanaian population showed that MMP-9 single nucleotide polymorphism 1562 C > T (rs3918242) was protective against placental malaria which suggests a possible role of MMP-9 in susceptibility to malaria.[34]
Dry eye
[edit]Dry eye patients, especially with meibomian gland dysfunction exhibit higher levels of MMP-9.[35]
References
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Further reading
[edit]- Nagase H, Woessner JF (July 1999). "Matrix metalloproteinases". The Journal of Biological Chemistry. 274 (31): 21491–4. doi:10.1074/jbc.274.31.21491. PMID 10419448.
- Zhao X, Wu T, Chang CF, Wu H, Han X, Li Q, Gao Y, Li Q, Hou Z, Maruyama T, Zhang J, Wang J (May 2015). "Toxic role of prostaglandin E2 receptor EP1 after intracerebral hemorrhage in mice". Brain, Behavior, and Immunity. 46: 293–310. doi:10.1016/j.bbi.2015.02.011. PMC 4422065. PMID 25697396.
- Starckx S, Van den Steen PE, Wuyts A, Van Damme J, Opdenakker G (February 2002). "Neutrophil gelatinase B and chemokines in leukocytosis and stem cell mobilization". Leukemia & Lymphoma. 43 (2): 233–41. doi:10.1080/10428190290005982. PMID 11999552. S2CID 940921.
- Wu H, Zhang Z, Li Y, Zhao R, Li H, Song Y, Qi J, Wang J (October 2010). "Time course of upregulation of inflammatory mediators in the hemorrhagic brain in rats: correlation with brain edema". Neurochemistry International. 57 (3): 248–53. doi:10.1016/j.neuint.2010.06.002. PMC 2910823. PMID 20541575.
- Bischof P, Meisser A, Campana A (2002). "Control of MMP-9 expression at the maternal-fetal interface". Journal of Reproductive Immunology. 55 (1–2): 3–10. doi:10.1016/S0165-0378(01)00142-5. PMID 12062817.
- St-Pierre Y, Van Themsche C, Estève PO (September 2003). "Emerging features in the regulation of MMP-9 gene expression for the development of novel molecular targets and therapeutic strategies". Current Drug Targets. Inflammation and Allergy. 2 (3): 206–15. doi:10.2174/1568010033484133. PMID 14561155. S2CID 453825.
- Wu H, Wu T, Hua W, Dong X, Gao Y, Zhao X, Chen W, Cao W, Yang Q, Qi J, Zhou J, Wang J (March 2015). "PGE2 receptor agonist misoprostol protects brain against intracerebral hemorrhage in mice". Neurobiology of Aging. 36 (3): 1439–50. doi:10.1016/j.neurobiolaging.2014.12.029. PMC 4417504. PMID 25623334.
- Lee JM, Yin K, Hsin I, Chen S, Fryer JD, Holtzman DM, Hsu CY, Xu J (March 2005). "Matrix metalloproteinase-9 in cerebral-amyloid-angiopathy-related hemorrhage". Journal of the Neurological Sciences. 229–230: 249–54. doi:10.1016/j.jns.2004.11.041. PMID 15760647. S2CID 21791281.
- Nair RR, Boyd DD (November 2005). "Expression cloning of novel regulators of 92 kDa type IV collagenase expression". Biochemical Society Transactions. 33 (Pt 5): 1135–6. doi:10.1042/BST20051135. PMID 16246065.
- Wu H, Zhang Z, Hu X, Zhao R, Song Y, Ban X, Qi J, Wang J (June 2010). "Dynamic changes of inflammatory markers in brain after hemorrhagic stroke in humans: a postmortem study". Brain Research. 1342: 111–7. doi:10.1016/j.brainres.2010.04.033. PMC 2885522. PMID 20420814.
- Wu H, Wu T, Han X, Wan J, Jiang C, Chen W, Lu H, Yang Q, Wang J (January 2017). "Cerebroprotection by the neuronal PGE2 receptor EP2 after intracerebral hemorrhage in middle-aged mice". Journal of Cerebral Blood Flow and Metabolism. 37 (1): 39–51. doi:10.1177/0271678X15625351. PMC 5363749. PMID 26746866.
- Ram M, Sherer Y, Shoenfeld Y (July 2006). "Matrix metalloproteinase-9 and autoimmune diseases". Journal of Clinical Immunology. 26 (4): 299–307. doi:10.1007/s10875-006-9022-6. PMID 16652230. S2CID 19619963.