MBAS assay: Difference between revisions
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A '''methylene blue active substances assay''', or '''MBAS assay''', is a [[colorimetric analysis]] [[test method]] that uses [[methylene blue]] to detect the presence of [[anionic]] [[surfactant]]s (such as a [[detergent]] or [[foaming agent]]) in a sample of [[water]]. |
A '''methylene blue active substances assay''', or '''MBAS assay''', is a [[colorimetric analysis]] [[test method]] that uses [[methylene blue]] to detect the presence of [[anionic]] [[surfactant]]s (such as a [[detergent]] or [[foaming agent]]) in a sample of [[water]]. An anionic surfactant detected by the [[color reaction]] is called a ''methylene blue active substance'' (MBAS).<ref>Alison L. George, Graham F. White "Optimization of the methylene blue assay for anionic surfactants added to estuarine and marine water" Environmental Toxicology and Chemistry 1999, Volume 18, pages 2232–2236. |
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{{DOI|10.1002/etc.5620181016}}</ref> |
{{DOI|10.1002/etc.5620181016}}</ref> |
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After first acidifying a water sample (with [[boric acid]], for example), one adds to it [[chloroform]] and a [[solution]] of methylene blue. Methylene blue is a [[cationic]] [[dye]]. The entire solution is then [[Agitator (device)|agitated]] to distribute these [[reagent]]s throughout the water sample. The color of the water changes according to the amount of surfactants in the sample. The higher the concentration of surfactants, the deeper and richer the blue coloration. |
After first acidifying a water sample (with [[boric acid]], for example), one adds to it [[chloroform]] and a [[solution]] of methylene blue et al.<ref name="EUR-Lex">[http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=CELEX:32004R0648:EN:NOT Regulation (EC) No 648/2004 of the European Parliament and of the Council of 31 March 2004 on detergents]</ref> Methylene blue is a [[cationic]] [[dye]]. The entire solution is then [[Agitator (device)|agitated]] to distribute these [[reagent]]s throughout the water sample.<ref name="EUR-Lex" /> The color of the water changes according to the amount of anionic surfactants in the sample. The higher the concentration of surfactants, the deeper and richer the blue coloration. |
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MBAS assay is |
MBAS assay is an [[ASTM International]] standard technique for detecting anionic surfactants.<ref name="astm">[http://www.astm.org/Standards/D2330.htm ASTM D2330 - 02 Standard Test Method for Methylene Blue Active Substances]</ref> These include [[carboxylate]]s, [[phosphate]]s, [[sulfate]]s, and [[sulfonate]]s. An MBAS assay alone does not, however, identify specific surfactants. ASTM withdrew the standard (ASTM D2330-02) in 2011 pending a review and update of the method, which was last approved in 2003.<ref name="astm" />. |
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==See also== |
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* [http://www.astm.org/Standards/D2330.htm ASTM D2330 - 02 Standard Test Method for Methylene Blue Active Substances] |
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* [http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=CELEX:32004R0648:EN:NOT Regulation (EC) No 648/2004 of the European Parliament and of the Council of 31 March 2004 on detergents] |
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==See also== |
==See also== |
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* [[Water testing]] |
* [[Water testing]] |
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{{Reflist}} |
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{{DEFAULTSORT:MBAS assay}} |
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[[Category:Analytical chemistry]] |
[[Category:Analytical chemistry]] |
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[[Category:Anionic surfactants]] |
[[Category:Anionic surfactants]] |
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Revision as of 18:52, 26 October 2011
A methylene blue active substances assay, or MBAS assay, is a colorimetric analysis test method that uses methylene blue to detect the presence of anionic surfactants (such as a detergent or foaming agent) in a sample of water. An anionic surfactant detected by the color reaction is called a methylene blue active substance (MBAS).[1]
After first acidifying a water sample (with boric acid, for example), one adds to it chloroform and a solution of methylene blue et al.[2] Methylene blue is a cationic dye. The entire solution is then agitated to distribute these reagents throughout the water sample.[2] The color of the water changes according to the amount of anionic surfactants in the sample. The higher the concentration of surfactants, the deeper and richer the blue coloration.
MBAS assay is an ASTM International standard technique for detecting anionic surfactants.[3] These include carboxylates, phosphates, sulfates, and sulfonates. An MBAS assay alone does not, however, identify specific surfactants. ASTM withdrew the standard (ASTM D2330-02) in 2011 pending a review and update of the method, which was last approved in 2003.[3].
See also
References
- ^ Alison L. George, Graham F. White "Optimization of the methylene blue assay for anionic surfactants added to estuarine and marine water" Environmental Toxicology and Chemistry 1999, Volume 18, pages 2232–2236. doi:10.1002/etc.5620181016
- ^ a b Regulation (EC) No 648/2004 of the European Parliament and of the Council of 31 March 2004 on detergents
- ^ a b ASTM D2330 - 02 Standard Test Method for Methylene Blue Active Substances
- Nollet, Leo M. L. Handbook of Water Analysis. 2nd ed. New York: Marcel Dekker, 2007.
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