Content deleted Content added
Line 38:
The process of abscission physically cleaves the midbody into two. Abscission proceeds by removal of cytoskeletal structures from the cytokinetic bridge, constriction of the cell cortex, and plasma membrane fission. The intercellular bridge is filled with dense bundles of antiparallel microtubules that derive from the central spindle. These microtubules overlap at the midbody, which is generally thought to be a targeting platform for the abscission machinery.
The microtubule severing protein [[spastin]] is largely responsible for the disassembly of microtubule bundles inside the intercellular bridge. Complete cortical constriction also requires removal of the underlying cytoskeletal structures. Actin filament disassembly during late cytokinesis depends on the PKCε–14-3-3 complex, which inactivates RhoA after furrow ingression. Actin disassembly is further controlled by the GTPase Rab35 and its effector, the phosphatidylinositol-4,5-bisphosphate 5-phosphatase OCRL. The final step of abscission is controlled by the recruitment and polymerization of the endosomal sorting complex required for transport III (ESCRT-III), which serves to physically constrict and separate the plasma membrane of the two adjoined daughter cells.<ref>Virginia Andrade et al. Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis. Sci. Adv.8 (2022). DOI:10.1126/sciadv.abm5095</ref>
===Timing cytokinesis===
| |||