Transcription-mediated amplification: Difference between revisions

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{{technical|date=May 2014}}
 
'''Transcription-mediated amplification''' ('''TMA''') is an isothermal (doesperformed not changeat the nucleic acidconstant temperature), single-tube [[nucleic acid]] [[Gene_duplication#As_amplification|amplification]] system utilizing two [[enzyme]]s, [[RNA polymerase]] and [[reverse transcriptase]].
 
"Amplification" means creating many more copies of a strand of nucleic acid than was present at first, in order to readily detect it or test it. Rapidly amplifying the target RNA/DNA allows a lab to simultaneously detect multiple pathogenic organisms in a single tube. TMA technology allows a clinical laboratory to perform [[nucleic acid test]] (NAT) assays for blood screening with fewer steps, less processing time, and faster results.