Transcriptome blood profile of the Yili horse before and after training

Li, Xueyan; Wang, Jianwen; Yao, Xinkui; Zeng, Yaqi; Wang, Chuankun; Ren, Wanlu; Yuan, Xinxin; Wang, Tongliang; Meng, Jun

doi:10.2754/avb202493020159

Acta Veterinaria > Archive > 2024, Vol. 93 > Issue 2 > Transcriptome blood profile of the Yili…

Acta Vet. Brno 2024, 93: 159-167

https://doi.org/10.2754/avb202493020159

Transcriptome blood profile of the Yili horse before and after training

Xueyan Li¹, Jianwen Wang^1,2,3, Xinkui Yao^1,2,3, Yaqi Zeng^1,2,3, Chuankun Wang¹, Wanlu Ren¹, Xinxin Yuan¹, Tongliang Wang¹, Jun Meng^1,2,3

¹Xinjiang Agricultural University, College of Animal Science, Urumqi City, Xinjiang Province, China
²Xinjiang Agricultural University, Horse Industry Research Institute, Urumqi City, Xinjiang Province, China
³Xinjiang Key Laboratory of Horse Breeding and Exercise Physiology, Urumqi City, Xinjiang Province, China

Received April 4, 2023
Accepted June 18, 2024

Proper training improves the horse’s performance. In the present study, blood transcriptome of the Yili horse was analysed before and after training. Overall, six Yili horses were trained for 3 months; blood samples at rest were collected before and after the training period. Transcriptome libraries were created from the blood samples using high-throughput sequencing; Gene Ontology functional enrichment and Kyoto Encyclopaedia of Genes and Genomes pathway analyses were performed for differentially expressed genes to identify the pathways and candidate genes associated with training and locomotor performance. In total, transcriptome analysis screened 33 significantly differentially expressed genes; of these, 23 and 10 were up-regulated and down-regulated after training, respectively. These differentially expressed genes were significantly enriched in metabolic pathways associated with exercise physiology, including sphingolipid metabolism, tryptophan metabolism, and the cGMP-PKG signalling pathway. The results of this study provide a theoretical basis for exploring the molecular mechanisms underlying locomotor performance of the Yili horse as well as a novel insight into the kinematics of this breed.

Keywords

Equine, daily training, differentially expressed genes.

Funding

Major science and technology projects of Xinjiang Uygur Autonomous Region” Construction of efficient breeding technology system for domestic horse specialized strains (Grant number 2022A02013-1), The Innovation Environment (Talent, Base) Construction Project of Xinjiang Uygur Autonomous Region (Grant number PT2220) , Key Laboratory of Xinjiang Uygur Autonomous Region Open Project. We acknowledge the support of the farm owners and veterinary practitioners.